Immunology and Cell Biology - Table 1 for article: Tracking membrane and secretory immunoglobulin [agr] heavy chain mRNA variation during B-cell differentiation by real-time quantitative polymerase chain reaction

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Tracking membrane and secretory immunoglobulin heavy chain mRNA variation during B-cell differentiation by real-time quantitative polymerase chain reaction

Sue D Xiang, Elizabeth M Benson and Ian S Dunn

Table 1. TaqMan primers and probes

Oligonucleotide name*	Function/reporter label^†	Sequence	GenBank Reference^‡
C/gen.F	Primer	CGCTGGCCTTCACACAGAA	J00220
C/Sec.R	Primer	CGCCATGACAACAGACACA	J00220
C/Mem.R	Primer	AGGGAGGCGGTATCTGCC	M85221
HPC1.F	Primer	AACCAATTTGGGTCACAGCTAAGTA	M57736
HPC1.R	Primer	TTCAGACCATCCATCAGCATACC	M57736
-actin.F	Primer	TCACCCACACTGTGCCCATCATCGA	X00351
-actin.R	Primer	CAGCGGAACCGCTCATTGCCAATGG	X00351
P1.C	Probe/FAM	CCATCGACCGCTTGGCGGG	J00220
P1.HPC1	Probe/FAM	CCTCAAGTCTGGCACATTTTTCTGGCC	M57736
P2.-actin	Probe/TET	ATGCCCTCCCCCATGCCATCCTGCGT	X00351

* Names denote target mRNA and directionality (F, forward; R, reverse). alpha s and alpha m both use a common probe (P1.C alpha ) and C alpha /gen.F as their common forward primer, with C alpha /Sec.R and C alpha /Mem.R as their reverse primers, respectively. P designates a probe oligonucleotide.

^† 5' reporter dyes for probe oligonucleotides as shown; all probes bear 3' phosphates and TAMRA quencher dyes.

^‡ Accession number for GenBank entry encompassing the oligonucleotide sequence.

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TABLE 1

Tracking membrane and secretory immunoglobulin heavy chain mRNA variation during B-cell differentiation by real-time quantitative polymerase chain reaction

Table 1. TaqMan primers and probes