FIGURE 2
FROM:
Tracking membrane and secretory immunoglobulin 
heavy chain mRNA variation during B-cell differentiation by real-time quantitative polymerase chain reaction
Sue D Xiang, Elizabeth M Benson and Ian S Dunn
BACK TO ARTICLEFigure 2.
Linear dynamic range of templates. The range of template RNA was from (a) 0.78 ng to 100 ng or (b) 25 ng to 500 ng at serial dilutions (1:2) for each data point, which represents the average of triplicate reactions. (a) 
s (
; primers C/gen-F + C/Sec-R; probe P1.C), m (
; primers C/gen-F + C/Mem-R; probe P1.C) and 
-actin (
; primers -actin-F + -actin-R; probe P2.-actin) expression in human tonsillar B cells. R2 values are 0.999, 0.998 and 0.997, respectively. (b) Human plasma cell antigen-1 (HPC1) (
; primers HPC1-F + HPC1-R; probe P1.HPC1) and -actin (; primers as for a) expression in human plasmacytoma cell line NCI-H929. R2 values are 0.993 and 0.997, respectively.
