Special Feature
Immunology and Cell Biology (1999) 77, 539–543; doi:10.1046/j.1440-1711.1999.00868.x
The use of carboxyfluorescein diacetate succinimidyl ester to determine the site, duration and cell type responsible for antigen presentation in vivo
Justine Mintern1,2, Ming Li3, Gayle M Davey1, Effrossini Blanas1, Christian Kurts1, Francis R Carbone3 and William R Heath1
- 1Immunology Division, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia
- 2The Cooperative Research Centre for Vaccine Technology, Queensland Institute of Medical Research, Herston, Queensland, Australia
- 3Department of Pathology and Immunology, Monash Medical School, Prahran, Victoria, Australia
Correspondence: William R Heath, Immunology Division, The Walter and Eliza Hall Institute, PO Royal Melbourne Hospital, Parkville, Vic. 3050, Australia. Email: heath@wehi.edu.au
Received 13 August 1999; Accepted 13 August 1999.
Abstract
This report examines the use of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) to determine the site, duration and cell type responsible for antigen presentation in vivo. Evidence that CFSE-labelled T cells can be used to determine where various types of antigens are presented, including auto-antigens, oral antigens and cell-associated foreign antigens, is provided. Using this technique, the length of time antigen is presented after acquisition by APC was measured. Finally, CFSE labelling was used to identify the origin of the APC responsible for different forms of antigen presentation.
Keywords:
antigen presentation, CD8-positive T lymphocytes, ovalbumin, transgenic mice
