FIGURE 5
FROM:
FGF signal transduction in PC12 cells: Comparison of the responses induced by endogenous and chimeric receptors
Erik D Foehr, Simona Raffioni, Ritsuko Fuji and Ralph A Bradshaw
BACK TO ARTICLEFigure 5.
Activation and association of signal transducers to platelet-derived growth factor (PDGF) in response to PDGF stimulation. (a) Rat phaeochromocytoma (PC12) cells expressing PFR1 were incubated at 37 °C with 30 ng/mL PDGF for 5 min. One thousand 
g of cell lysate was incubated with PDGFR antibody, collected by Protein A-Sepharose and subjected to 7.5% SDS-PAGE and western blot analysis. After probing, the blot was stripped and reprobed as indicated. Panel 1, blot probed with anti-phosphotyrosine antibody; panel 2, blot probed with PDGFR antibody; panel 3, blot probed with PLC
antibody. (b) PC12 cells expressing PFR1 were incubated at 37 °C with 30 ng/mL PDGF for 5 min. One thousand g of cell lysate was incubated with Shc antisera, collected by Protein A-Sepharose and subjected to 10% SDS-PAGE and western blot analysis. Panel 1, blot probed with anti-phosphotyrosine antibody; panel 2, blot probed with Grb2 antibody.
