FIGURE 1

Expression of fibroblast growth factor receptor FGFR1, 3 and 4 mRNA in rat phaeochromocytoma (PC12) cells. Poly (A)⁺ mRNA (3 g/lane) was isolated from PC12 cells and subjected to northern blot analysis by probing it with [-³²P]dCTP-labelled cDNA corresponding to a portion of the extracellular domain of PC12 FGFR1 (a), FGFR3 (b) and FGFR4 (c). The blot in (a) was stripped and reprobed with CHOb to normalize RNA loading differences. The arrows point to the major transcript of each FGFR. An RNA ladder was used for molecular size standard and is shown on the left. The autoradiographs shown were obtained after exposing to X-ray films for 8 h (a) and 48 h (b and c).