Original Article

Hypertension Research (2005) 28, 711–718; doi:10.1291/hypres.28.711

Antihypertensive Effect of Green Coffee Bean Extract on Mildly Hypertensive Subjects

Kazuya Kozuma, Shigemi Tsuchiya, Jun Kohori, Tadashi Hase and Ichiro Tokimitsu

Health Care Research Laboratories, Kao Corporation, Tokyo, Japan

Correspondence: Kazuya Kozuma, Ph.D., Health Care Research Laboratories, Kao Corporation, 2-1-3 Bunka, Sumida-ku, Tokyo 131-8501, Japan. E-mail: kouzuma.kazuya@kao.co.jp

Received 1 March 2005; Accepted 27 June 2005.

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Abstract

A water-soluble green coffee bean extract (GCE) has been shown to be effective against hypertension in both spontaneously hypertensive rats and humans. This multicenter, randomized, double-blind, placebo-controlled, parallel group study evaluated the dose-response relationship of GCE in 117 male volunteers with mild hypertension. Subjects were randomized into four groups: a placebo and three drug groups that received 46 mg, 93 mg, or 185 mg of GCE once a day. After 28 days, systolic blood pressure (SBP) in the placebo, 46 mg, 93 mg, and 185 mg groups was reduced by -1.3plusminus3.0 mmHg, -3.2plusminus4.6 mmHg, -4.7plusminus4.5 mmHg, and -5.6plusminus4.2 mmHg from the baseline, respectively. The decreases in SBP in the 93 mg group (p<0.05) and the 185 mg group (p<0.01) were statistically significant compared with the placebo group. Diastolic blood pressure (DBP) in the placebo, 46 mg, 93 mg, and 185 mg groups was reduced by -0.8plusminus3.1 mmHg, -2.9plusminus2.9 mmHg, -3.2plusminus3.2 mmHg, and -3.9plusminus2.8 mmHg from the baseline, respectively, and significant effects were observed in the 93 mg group (p<0.05) and the 185 mg group (p<0.01) compared with the placebo group. Both blood pressures were significantly reduced in a dose-related manner by GCE (p<0.001). Adverse effects caused by GCE were not observed. The results suggested that daily use of GCE has a blood pressure-lowering effect in patients with mild hypertension.

Keywords:

hypertension, chlorogenic acid, dose-response, human, randomized controlled study

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References

  1. Eastern Stroke and Coronary Heart Disease Collaborative Research Group: Blood pressure, cholesterol, and stroke in eastern Asia. Lancet 1998; 352: 1801–1807. | ISI |
  2. Domanski M, Mitchell G, Pfeffer M, et al: Pulse pressure and cardiovascular disease-related mortality: follow-up study of the Multiple Risk Factor Intervention Trial (MRFIT). JAMA 2002; 287: 2677–2683. | Article | PubMed | ISI |
  3. Lloyd-Jones DM, Larson MG, Leip EP, et al: Lifetime risk for developing congestive heart failure: the Framingham Heart Study. Circulation 2002; 106: 3068–3072. | Article | PubMed |
  4. Whelton PK, He J, Appel LJ, et al, National High Blood Pressure Education Program Coordinating Committee: Primary prevention of hypertension: clinical and public health advisory from the National High Blood Pressure Education Program. JAMA 2002; 288: 1882–1888. | Article | PubMed | ISI |
  5. Japanese Society of Hypertension Guidelines Subcommittee for the Management of Hypertension: Guidelines for the management of hypertension for general practitioners. Hypertens Res 2001; 24: 613–634. | ISI |
  6. FitzGerald RJ, Murray BA, Walsh DJ: Hypotensive peptides from milk proteins. J Nutr 2004; 134: 980S–988S. | PubMed | ChemPort |
  7. Block G, Mangels AR, Norkus EP, Patterson BH, Levander OA, Taylor PR: Ascorbic acid status and subsequent diastolic and systolic blood pressure. Hypertension 2001; 37: 261–267. | PubMed | ChemPort |
  8. Noguchi T, Ikeda K, Sasaki Y, et al: Effects of vitamin E and sesamin on hypertension and cerebral thrombogenesis in stroke-prone spontaneously hypertensive rats. Hypertens Res 2001; 24: 735–742. | Article | PubMed | ChemPort |
  9. Mullan BA, Young IS, Fee H, MaCance DR: Ascorbic acid reduces blood pressure and arterial stiffness in type 2 diabetes. Hypertension 2002; 40: 804–809. | Article | PubMed | ChemPort |
  10. Boshtam M, Rafiei M, Sadeghi K, Sarraf-Zadegan N: Vitamin E can reduce blood pressure in mild hypertensives. Int J Vitam Nutr Res 2002; 72: 309–314. | Article | PubMed | ChemPort |
  11. Galley HE, Thornton J, Howdle PD, Walker BE, Webster NR: Combination oral antioxidant supplementation reduces blood pressure. Clin Sci 1997; 92: 361–365. | PubMed | ChemPort |
  12. Seppo L, Jauhiaine T, Poussa T, Korpela R: A fermented milk high in bioactive peptides has a blood pressure-lowering effect in hypertensive subjects. Am J Clin Nutr 2003; 77: 326–330. | PubMed | ChemPort |
  13. Kawasaki T, Seki E, Osajima K, et al: Antihypertensive effect of valyl-tyrosine, a short chain peptide derived from sardine muscle hydrolyzate, on mild hypertensive subjects. J Hum Hypertens 2000; 14: 519–523. | Article | PubMed | ChemPort |
  14. Das UN: Long-chain polyunsaturated fatty acids interact with nitric oxide, superoxide anion, and transforming growth factor-beta to prevent human essential hypertension. Eur J Clin Nutr 2004; 58: 195–203. | Article | PubMed | ChemPort |
  15. Gokce N: L-Arginine and hypertension. J Nutr 2004; 134: 2807S–2811S. | PubMed | ChemPort |
  16. Iseki K, Iseki C, Itoh K, et al: Estimated protein intake and blood pressure in a screened cohort in Okinawa, Japan. Hypertens Res 2003; 26: 289–294. | Article | PubMed | ChemPort |
  17. Hermansen K: Diet, blood pressure and hypertension. Br J Nutr 2000; 83: S113–S119. | Article | PubMed | ChemPort |
  18. Saltzman E, Das SK, Lichtenstein AH, et al: An oat-containing hypocaloric diet reduces systolic blood pressure and improves lipid profile beyond effects of weight loss in men and women. J Nutr 2001; 131: 1465–1470. | PubMed | ChemPort |
  19. Burke V, Hodgson JM, Beilin LJ, Giangiulioi N, Rogers P, Puddey IB: Dietary protein and soluble fiber reduce ambulatory blood pressure in treated hypertensives. Hypertension 2001; 38: 821–826. | Article | PubMed | ChemPort |
  20. Kondo S, Tayama K, Tsukamoto Y, Ikeda K, Yamori Y: Antihypertensive effects of acetic acid and vinegar on spontaneously hypertensive rats. Biosci Biotechnol Biochem 2001; 65: 2690–2694. | Article | PubMed | ChemPort |
  21. Jenkins DJ, Kendall CW, Jackson CJ, et al: Effects of high- and low-isoflavone soyfoods on blood lipids, oxidized LDL, homocysteine, and blood pressure in hyperlipidemic men and women. Am J Clin Nutr 2002; 76: 365–372. | PubMed | ISI | ChemPort |
  22. Rivas M, Garay RP, Escanero JF, Cia PJ, Cia P, Alda JO: Soy milk lowers blood pressure in men and women with mild to moderate essential hypertension. J Nutr 2002; 132: 1900–1902. | PubMed | ChemPort |
  23. Taubert D, Berkels R, Roesen R, Klaus W: Chocolate and blood pressure in elderly individuals with isolated systolic hypertension. JAMA 2003; 290: 1029–1030. | Article | PubMed |
  24. Aoki H, Furuya Y, Endo Y, Fujimoto K: Effect of gamma-aminobutyric acid-enriched Tempeh-like fermented soybean (GABA-Tempeh) on the blood pressure of spontaneously hypertensive rats. Biosci Biotechnol Biochem 2003; 67: 1806–1808. | Article | PubMed | ChemPort |
  25. Suzuki A, Kagawa D, Ochiai R, Tokimitsu I, Saito I: Green coffee bean extract and its metabolites have a hypotensive effect in spontaneously hypertensive rats. Hypertens Res 2002; 25: 99–107. | Article | PubMed | ChemPort |
  26. Saito I, Tsuchida T, Watanabe T, et al: Effect of coffee bean extract in essential hypertension. Jpn J Med Pharm Sci 2002; 47: 67–74 (in Japanese).
  27. Olthof MR, Hollman PC, Michel N, et al: Chlorogenic acid, quercetin-3-rutinoside and black tea phenols are extensively metabolized in humans. J Nutr 2003; 133: 1806–1814. | PubMed | ChemPort |
  28. Ochiai R, Jokura H, Suzuki A, et al: Green coffee bean extract improves human vasoreactivity. Hypertens Res 2004; 27: 731–737. | Article | PubMed | ChemPort |
  29. Olthof MR, Hollman PC, Katan MB: Chlorogenic acid and caffeic acid are absorbed in humans. J Nutr 2001; 131: 66–71. | PubMed | ChemPort |
  30. Suzuki A, Kagawa D, Fujii A, Ochiai R, Tokimitsu I, Saito I: Short- and long-term effects of ferulic acid on blood pressure in spontaneously hypertensive rats. Am J Hypertens 2002; 15: 351–357. | Article | PubMed | ChemPort |
  31. Nakazono K, Watanabe N, Matsuno K, Sasaki J, Sato T, Inoue M: Dose superoxide underlie the pathogenesis of hypertension? Proc Natl Acad Sci U S A 1991; 88: 10045–10048. | Article | PubMed | ChemPort |
  32. Romero JC, Reckelhoff JF: Role of angiotensin and oxidative stress in essential hypertension. Hypertension 1999; 34: 943–949. | PubMed | ISI | ChemPort |
  33. Hozawa A, Ebihara S, Ohmori K, et al: Increased plasma 8-isoprostane levels in hypertensive subjects: the Tsurugaya Project. Hypertens Res 2004; 27: 557–561. | Article | PubMed | ChemPort |
  34. Redon J, Oliva MR, Tormos C, et al: Antioxidant activities and oxidative stress byproducts in human hypertension. Hypertension 2003; 41: 1096–1101. | Article | PubMed | ISI | ChemPort |
  35. Taddei S, Virdis A, Ghiadoni L, Magagna A, Salvetti A: Vitamin C improves endothelium-dependent vasodilation by restoring nitric oxide activity in essential hypertension. Circulation 1998; 97: 2222–2229. | PubMed | ISI | ChemPort |
  36. Hirooka Y, Eshima K, Setoguchi S, et al: Vitamin C improves attenuated angiotensin II-induced endothelium-dependent vasodilation in human forearm vessels. Hypertens Res 2003; 26: 953–959. | Article | PubMed | ChemPort |
  37. Kono Y, Shibata H, Kodama Y, Ueda A, Sawa Y: Chlorogenic acid as a natural scavenger for hypochlorous acid. Biochem Biophys Res Commun 1995; 217: 972–978. | Article | PubMed | ChemPort |
  38. Sawa T, Nakao M, Akaike T, Ono K, Maeda H: Alkylperoxyl radical scavenging activity of various flavonoids and anti-tumor-promoter effect of vegetables. J Agric Food Chem 1999; 47: 397–402. | Article | PubMed | ISI | ChemPort |
  39. Nakatani N, Kayano S, Kikuzaki H, Sumino K, Katagiri K, Mitani T: Identification, quantitative determination, and antioxidative activities of chlorogenic acid isomers in prune (Prunus domestica L). J Agric Food Chem 2000; 48: 5512–5516. | Article | PubMed | ChemPort |
  40. Son S, Lewis BA: Free radical scavenging and antioxidative activity of caffeic acid amide and ester analogues: structure-activity relationship. J Agric Food Chem 2002; 50: 468–472. | Article | PubMed | ChemPort |
  41. Kikuzaki H, Hisamoto M, Hirose K, Akiyama K, Taniguchi H: Antioxidant properties of ferulic acid and its related compounds. J Agric Food Chem 2002; 50: 2161–2168. | Article | PubMed | ChemPort |
  42. Iwai K, Kishimoto N, Kakino Y, Mochida K, Fujita T: In vitro antioxidative effects and tyrosinase inhibitory activities of seven hydroxycinnamoyl derivatives in green coffee beans. J Agric Food Chem 2004; 52: 4893–4898. | Article | PubMed | ChemPort |
  43. Midgley JP, Matthew AG, Greenwood CM, Logan AG: Effect of reduced dietary sodium on blood pressure: a meta-analysis of randomized controlled trials. JAMA 1996; 275: 1590–1597. | Article | PubMed | ISI | ChemPort |
  44. Whelton PK, Appel LJ, Espeland MA, et al, TONE Collaborative Research Group: Sodium reduction and weight loss in the treatment of hypertension in older persons: a randomized controlled trial of nonpharmacologic interventions in the elderly (TONE). JAMA 1998; 279: 839–846. | Article | PubMed | ISI | ChemPort |
  45. Vollmer WM, Sacks FM, Ard J, et al, DASH-Sodium Trial Collaborative Research Group: Effects of diet and sodium intake on blood pressure: subgroup analysis of the DASH-sodium trial. Ann Intern Med 2001; 135: 1019–1028. | PubMed | ISI | ChemPort |
  46. O'Shaughnessy KM, Karet FE: Salt handling and hypertension. J Clin Invest 2004; 113: 1075–1081. | Article | PubMed | ChemPort |
  47. Obarzanek E, Proschan MA, Vollmer WM, et al: Individual blood pressure responses to changes in salt intake: results from the DASH-Sodium trial. Hypertension 2003; 42: 459–467. | Article | PubMed | ISI | ChemPort |
  48. Nishijima K, Tochikubo O: Sodium intake and cardiac sympatho-vagal balance in young men with high blood pressure. Hypertens Res 2004; 27: 393–398. | Article | PubMed |
  49. MacMahon S, Peto R, Cutler J, et al: Blood pressure, stroke, and coronary heart disease. Part 1, Prolonged differences in blood pressure: prospective observational studies corrected for the regression dilution bias. Lancet 1990; 335: 765–774. | Article | PubMed | ISI | ChemPort |