TABLE 3
FROM:
Recombination and clonal propagation in different populations of the lichen Lobaria pulmonaria
J-C Walser, F Gugerli, R Holderegger, D Kuonen and C Scheidegger
BACK TO ARTICLETable 3. Microsatellite loci of Lobaria pulmonaria included in this study
| Locus | Primer sequence (5'–3') | Ta (°C) | Label | Allele size range (bp) | Number of alleles |
|---|---|---|---|---|---|
| LPu03 | F: GGCTGCAATGATGACTAGGAR: CACCCTGGTGTTGACTGCTA | 55 | NED | 187–193 | 4 |
| LPu09 | F: AGCCTGGAGTTCAGACAACC
R: ATCTTGTCTTGGCGCTTCTG | 55 | 6FAM | 181–640 | 34 |
| LPu15 | F: CAAAATACCTGAATGGATGT
R: CTGAGGCAACACTCTACAGC | 55 | HEX | 149–203 | 20 |
| LPu16 | F: GCCTGCCAAAGAATACAGCA
R: TGTCGATGTCTTGCCTGAAC | 57 | HEX | 186–260 | 23 |
| LPu20 | F: CTCTGCCGCTCGGGTTACAT
R: TGCCAGTACTGCAATGTGGT | 57 | NED | 161–241 | 32 |
| LPu27 |
F: GCTCATGCTCCACATCTGACGCTCATGCTCCACATCTGAC R: CATGCTCTTCCATTCACAGC | 57 | 6FAM | 170–206 | 14 |
Primer sequence (F: forward; R: reverse), annealing temperature (T a) in multiplex-PCR, dye used for 5' fluorescent labelling of forward primer, range of allele sizes, and number of alleles detected. The six loci are described in Walser et al (2003), but four primers (given in bold) were re-designed in order to shorten allele sizes or to avoid indels in the flanking regions.
