Original Article

Heredity (1996) 77, 619–628; doi:10.1038/hdy.1996.189

Local genetic structure within two rookeries of Chelonia mydas (the green turtle)

Tigerin Peare1 and Patricia G Parker1

1Department of Zoology, Ohio State University, Columbus, OH 43210, USA

Correspondence: Tigerin Peare, Department of Zoology, Ohio State University, Columbus, OH 43210, USA

Received 26 February 1996.

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Abstract

We used multilocus minisatellite DNA fingerprinting to examine the local genetic structure within nesting populations of green turtles (Chelonia mydas) in Tortuguero, Costa Rica and Melbourne, Florida, USA. In the Tortuguero population, there was a significant negative correlation between genetic similarity of pairs of nesting females and the distance between their nest sites both within years (r2=0.273; P<0.001) and between years (r2=0.578; P<0.001). Of the 122 female pairs scored for Tortuguero, 12.3 per cent had genetic similarity values resembling those of mother-offspring pairs. In the Melbourne population, however, no relationship between genetic similarity and distance was found (r2=0.017; P=0.075). The distance-related genetic structure of the Tortuguero population indicates that these females exhibit low levels of dispersal from natal sites, and that nestmates return independently to nest near their natal sites. The lack of a similar structure in the Melbourne population suggests that females from this population may not return to natal sites with comparable precision. High levels of mortality among nests, hatchlings or maturing turtles produced in the Melbourne rookery may also be responsible for the absence of distance-related local genetic structure.

Keywords:

Chelonia mydas, DNA fingerprinting, genetic structure, philopatry

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References

  1. Allard, M W, Miyamoto, M M, Bjorndal, K A, Bolten, A B, and Bowen, B W. 1994. Support for natal homing in green turtles from mitochondrial DNA sequences. Copeia, 1994, 34–41.
  2. Antlfinger, A E. 1981. The genetic basis of microdifferentiation in natural and experimental populations of Borrichia frutescens in relation to salinity. Evolution, 35, 1056–1069.
  3. Antonovics, J, and Bradshaw, A D. 1970. Evolution in closely adjacent plant populations. VIII. Clinal patterns at a mine boundary. Heredity, 25, 349–362. | ISI |
  4. Barrowclough, G F, and Coats, S L. 1985. The demography and population genetics of owls, with special reference to the conservation of the spotted owl (Strix occidentalis). In: Gutierrez, R. J and Carey, A. B. (eds) Ecology and Management of the Spotted Owl in the Pacific Northwest, pp. 74–85. US Forest Service, Portland, OR.
  5. Bishop, J A. 1981. A neo-Darwinian approach to resistance: examples from mammals. In: Bishop, J. A and Cook, L. M. (eds) Genetic Consequences of Man-Made Change, pp. 37–51. Academic Press, London.
  6. Bishop, J A, and Hartley, D J. 1976. The size and age structure of rural populations of Rattus norvegicus containing individuals resistant to the anticoagulant poison warfarin. J Anim Ecol, 45, 623–646.
  7. Bishop, J A, Hartley, D J, and Partridge, G G. 1977. The population dynamics of genetically determined resistance to warfarin in Rattus norvegicus from mid Wales. Heredity, 39, 389–398.
  8. Bjorndal, K. 1982. The consequences of herb ivory for the life history pattern of the Caribbean green turtle, Chelonia mydas. In: Bjorndal, K. (ed.) Biology and Conservation of Sea Turtles, pp. 19–26. Smithsonian Institution Press, Washington, DC.
  9. Bowen, B W, Meylan, A B, Ross, J P, Limpus, C J, Balazs, G H, and Avise, J C. 1992. Global population structure and natural history of the green turtle (Chelonia mydas) in terms of matriarchal phylogeny. Evolution, 46, 865–881.
  10. Burke, T, and Bruford, M W. 1987. DNA fingerprinting in birds. Nature, 327, 149–152. | Article | PubMed | ISI | ChemPort |
  11. Carr, A. 1967. So Excellente a Fishe. Natural History Press, New York.
  12. Carr, A. 1980. Some problems of sea turtle ecology. Am Zool, 20, 489–498.
  13. Carr, A. 1982. Notes on the behavioral ecology of sea turtles. In: Bjorndal, K. (ed.) Biology and Conservation of Sea Turtles, pp. 19–26. Smithsonian Institution Press, Washington, DC.
  14. Carr, A, and Carr, M H. 1972. Site fixity in the Caribbean green turtle. Ecology, 53, 425–429.
  15. Carr, A, and Hirth, H. 1962. The ecology and migrations of sea turtles, V: Comparative features of isolated green turtle colonies. Am Mus Novit, 2091, 1–42.
  16. Carr, A, and Ogren, L. 1960. The ecology and migrations of sea turtles IV: The green turtle in the Caribbean Sea. Bull Am Mus Nat Hist, 121, 1–48.
  17. Carr, A, Carr, M H, and Meylan, A B. 1978. The ecology and migrations of sea turtles. 7. The west Caribbean green turtle colony. Bull Am Mus Nat Hist, 162, 1–46.
  18. Chapman, R W. 1989. Spatial and temporal variation of mitochondrial DNA haplotype frequencies in the striped bass (Morone saxatilis) 1982 year class. Copeia, 1989, 344–348.
  19. Decker, M D, Parker, P G, Minchella, D J, and Rabenold, K N. 1993. Monogamy in black vultures: genetic evidence from DNA fingerprinting. Behav Ecol, 4, 29–35.
  20. Ehrlich, P R, and Raven, P H. 1969. Differentiation of populations. Science, 165, 1227–1232.
  21. Frazer, N B, and Ladner, R C. 1986. A growth curve for green turtles (Chelonia mydas) in the U.S. Virgin Islands. Copeia, 1986, 798–802.
  22. Georges, M, Lequarre, A-S, Castelli, M, Hanset, R, and Vassart, G. 1988. DNA fingerprinting in domestic animals using four different minisatellite probes. Cytogenet Cell Genet, 47, 127–131. | PubMed | ISI | ChemPort |
  23. Gilbert, D A, Lehman, N, O'Brien, S J, and Wayne, R K. 1990. Genetic fingerprinting reflects population differentiation in the California Channel Island fox. Nature, 344, 764–766. | Article | PubMed | ChemPort |
  24. Jain, S K, and Bradshaw, A D. 1966. Evolutionary divergence among adjacent plant populations. I. The evidence and its theoretical analysis. Heredity, 20, 407–441.
  25. Jeffreys, A J, Wilson, V, and Thein, S L. 1985. Hypervariable "minisatellite" regions in human DNA. Nature, 314, 67–73. | Article | PubMed | ISI | ChemPort |
  26. Johnson, S. 1994. Reproductive Ecology of the Florida Green Turtle (Chelonia mydas). M.Sc. Thesis, University of Florida.
  27. Koch, A L, Carr, A F, and Ehrenfeld, D W. 1969. The problem of open-sea navigation: the migration of the green sea turtle to Ascension Island. J Theor Biol, 22, 163–179. | Article | PubMed | ChemPort |
  28. Lohmann, K J. 1992. How sea turtles navigate. Sci Am, 266, 100–106.
  29. Longmire, J L, Lewis, A W, Brown, N C, Buckingham, J M, Clark, L M, Jones, M D. et al. 1988. Isolation and molecular characterization of a highly polymorphic centromeric tandem repeat in the family Falconidae. Genomics, 2, 14–24.
  30. Lynch, M. 1990. The similarity index and DNA fingerprinting. Mol Biol Evol, 7, 478–484. | PubMed |
  31. Mantel, N. 1967. The detection of disease clustering and a generalized regression approach. Cancer Res, 27, 209–220. | PubMed | ISI | ChemPort |
  32. Meylan, A B, Bowen, B W, and Avise, J C. 1990. A genetic test of the natal homing versus social facilitation models for green turtle migration. Science, 248, 724–727. | PubMed | ChemPort |
  33. Mortimer, J A. 1982. Factors affecting beach selection by nesting sea turtles. In: Bjorndal, K. (ed.) Biology and Conservation of Sea Turtles, pp. 45–51. Smithsonian Institution Press, Washington, DC.
  34. Mortimer, J A, and Portier, K M. 1989. Reproductive homing and internesting behavior of the green turtle (Chelonia mydas) at Ascension Island, South Atlantic Ocean. Copeia, 1989, 962–977.
  35. Parker, P G, and Whiteman, H. 1993. Genetic diversity in fragmented populations of Clemmys guttata and Chrysemys picta marginata as shown by DNA fingerprinting. Copeia, 1993, 841–846.
  36. Piper, W H, and Parker Rabenold, P. 1992. Use Of fragment-sharing estimates from DNA fingerprinting to determine relatedness in a tropical wren. Mol Ecol, 1, 69–78.
  37. Rabenold, P P, Rabenold, K N, Piper, W H, Haydock, J H, and Zack, S W. 1990. Shared paternity revealed by genetic analysis in cooperatively breeding tropical wrens. Nature, 348, 538–540. | Article | ChemPort |
  38. Rohlf, F J. 1990. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System. Version 1.60. Applied Biostatistics, Setauket, New York.
  39. Rohlf, F J, and Schnell, G D. 1971. An investigation of the isolation-by-distance model. Am Nat, 105, 245–324.
  40. Schnell, G D, Watt, D J, and Douglas, M E. 1985. Statistical comparison of proximity matrices: applications in animal behaviour. Anim Behav, 33, 239–253.
  41. Selander, R K. 1970. Behavior and genetic variation in natural populations. Am Zool, 10, 53–66. | PubMed | ChemPort |
  42. Snaydon, R W, and Davies, M S. 1972. Rapid population differentiation in a mosaic environment. II. Morphological variation in Anthoxanthum odoratum. Evolution, 26, 390–405.
  43. Sokal, R R, and Wartenberg, D E. 1983. A test of spatial autocorrelation analysis using an isolation-by-distance model. Genetics, 105, 219–237. | PubMed | ISI |
  44. Southern, E M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol, 98, 503–517. | Article | PubMed | ISI | ChemPort |
  45. Triggs, S J, Williams, M J, Marshall, S J, and Chambers, G K. 1992. Genetic structure of blue duck (Hymenolaimus malcorhynchos) populations revealed by DNA fingerprinting. Auk, 109, 80–89.
  46. Weider, L J. 1985. Spatial and temporal genetic heterogeneity in a natural Daphnia population. J Plankton Res, 7, 101–123.
  47. White, M M, and Svendsen, G E. 1992. Spatial-genetic structure in the easten chipmunk, Tamias striatus. J Mammal, 73, 619–624.
  48. Witherington, B E. 1992. Behavioral responses of nesting sea turtles to artificial lighting. Herpetologica, 48, 31–39.
  49. Worth, D F, and Smith, J B. 1976. Marine turtle nesting on Hutchinson Island, Florida, in 1973. Florida Mar Res Publ, 18, 1–17.
  50. Wright, S. 1943a. Isolation by distance. Genetics, 28, 114–138. | PubMed | ISI | ChemPort |
  51. Wright, S. 1943b. An analysis of local variability of flower color in Linanthus parryae. Genetics, 28, 139–156.
  52. Wright, S. 1951. The genetical structure of populations. Ann Eugen, 15, 323–354. | ISI |