Original Article

Heredity (1996) 77, 433–438; doi:10.1038/hdy.1996.163

On the difficulties of discriminating between major and minor hybrid male sterility factors in Drosophila by examining the segregation ratio of sterile and fertile sons in backcrossing experiments

Xulio R Maside1 and Horacio F Naveira1

1Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de La Coruña, Campus da Zapateira, La Coruña, Spain

Correspondence: Horacio F Naveira, Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de La Coruña, Campus da Zapateira, La Coruña, Spain

Received 28 November 1995.

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Abstract

The oberservation of segregation ratios of sterile and fertile males in offspring samples from backcrossed hybrid females is, in principle, a valid method to unveil the genetic basis of hybrid male sterility in Drosophila. When the female parent is heterozygous (hybrid) for a sterility factor with major effects, equal proportions of fertile and sterile sons are expected in her offspring. However, intact (not recombined) chromosome segments of considerable length are expected to give segregation ratios that can not be easily differentiated from the 1:1 ratio expected from a single factor. When the phenotypic character under analysis can be determined by combinations of minor factors from the donor species spanning a certain chromosome length, very large offspring samples may be needed to test this alternative hypothesis against the null hypothesis of a single major factor. This is particularly the case of hybrid male sterility determinants in Drosophila.

Keywords:

Drosophila, hybrid males, major genes, polygenes, recombination, sterility

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References

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