Original Article

Heredity (1995) 75, 297–302; doi:10.1038/hdy.1995.138

Chromosomal location of the amplified esterase genes conferring resistance to insecticides in Myzus persicae (Homoptera: Aphididae)

R L Blackman1, J M Spence1, L M Field2 and A L Devonshire2

  1. 1Department of Entomology, The Natural History Museum, Cromwell Road, London SW7 5BD, UK
  2. 2Biological and Ecological Chemistry Department, IACR-Rothamsted, Harpenden AL5 2JQ, UK

Correspondence: RL Blackman, Department of Entomology, The Natural History Museum, Cromwell Road, London SW7 5BD, UK

Received 16 January 1995.

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Abstract

A genomic probe encompassing most of an esterase gene (E4) that is amplified in insecticide-resistant Myzus persicae was hybridized in situ to mitotic and meiotic chromosome preparations of aphid clones of known esterase type and resistance level. Binding, which was detected using the biotin-avidin system located both known types of amplified esterase sequences (E4 and FE4). All except one of the E4-producing clones had a single amplified site, on autosome 3 near the breakpoint of an autosomal 1,3 translocation which previous work had shown to be genetically linked to insecticide resistance. The exceptional clone had two other E4-encoding sites. The most resistant FE4-producing clone (800F) had amplified sequences at five sites (three loci: two homozygous and one heterozygous). Altogether, amplified E4 and/or FE4 sequences were found on four of the five autosome pairs of M. persicae. Possible origins of these multiple loci are discussed.

Keywords:

aphid chromosomes, esterase genes, FISH gene amplification, insecticide resistance, position effect

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References

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