Original Article

Heredity (1995) 74, 210–215; doi:10.1038/hdy.1995.29

Genetics of aliphatic glucosinolates. III. Side chain structure of aliphatic glucosinolates in Arabidopsis thaliana

R Mithen1, J Clarke2, C Lister2 and C Dean2

  1. 1Brassica and Oilseeds Research Department, John Innes Centre, Colney Lane, Norwich NR4 7UJ, UK
  2. 2Molecular Genetics Department, John Innes Centre, Colney Lane, Norwich NR4 7UJ, UK

Correspondence: R Mithen, Brassica and Oilseeds Research Department, John Innes Centre, Colney Lane, Norwich NR4 7UJ, UK

Received 28 June 1994.

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Abstract

The inheritance of aliphatic glucosinolates in Arabidopsis thaliana was studied. Analysis of F3 families from a cross between the ecotype Limburg-5 and the accession H51 (an inbred line derived from the ecotype Stockholm) suggested that alleles at a single locus regulate the conversion of methylsulphinylalkyl glucosinolates into alkenyl (and hydroxyalkenyl) glucosinolates. Likewise, analysis of recombinant inbred lines derived from a cross between the ecotypes Columbia and Landsberg erecta suggested that alleles at a single locus regulate the conversion of methylsulphinylpropyl glucosinolate into hydroxypropyl glucosinolate. Both loci mapped to a similar position on chromosome 4 and it is suggested that these alleles occur at the same locus. A genetic model is proposed in which the aliphatic glucosinolates of A. thaliana are determined by alleles at three loci. The ecological significance of variation in aliphatic glucosinolates is discussed.

Keywords:

Arabidopsis thaliana, gene mapping, genetic diversity, glucosinolates, pests

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References

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