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  • Viral Transfer Technology
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Baculovirus-mediated periadventitial gene transfer to rabbit carotid artery

Gene Therapy volume 7, pages 1499–1504 (2000)Cite this article

Abstract

Recombinant Autographa californica multiple nuclear polyhedrosis viruses (AcMNPV) have recently been shown to transduce mammalian cells in vitro. Since baculoviruses offer many advantages over viruses currently used in gene therapy, we have tested them for in vivo gene transfer by constructing a baculovirus bearing a nuclear targeted β-galactosidase marker gene (LacZ) under a CMV promoter. Both rabbit aortic smooth muscle cells (RAASMC) and human ECV-304 cells were susceptible to LacZ-baculovirus transduction. Transgene expression was evaluated in vivo by applying 1 × 109 p.f.u. of LacZ-baculoviruses or LacZ-adenoviruses in a silastic collar placed around rabbit carotid arteries in the absence of contact with blood components. As a result, baculoviruses led to transgene expression in adventitial cells in rabbit carotid arteries with efficiency comparable to adenoviruses. The β-galactosidase gene expression was transient staying at a high level for 1 week but disappearing at the 14 day time-point. The arterial structure and endothelium remained intact in the baculovirus-transduced arteries, but macrophage-specific immunostaining detected signs of inflammation comparable to adenoviruses. Baculoviruses are thus able to mediate transient gene transfer in vivo and may become useful tools for gene therapy.

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Acknowledgements

We thank Ms Irene Helkala, Ms Maiju Jääskeläinen, Ms Mervi Nieminen, Ms Eila Pelkonen, Ms Mervi Riekkinen, and Ms Mari Supinen for expert technical assistance, and Ms Marja Poikolainen for preparing the manuscript. This study was supported by grants from the Academy of Finland, European Union Biomed Program (BMH4 CT-95-0329) and Kuopio University Hospital (EVO grant 5130).

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  1. M O Hiltunen and M P Turunen: Both authors contributed equally

Authors and Affiliations

  1. Department of Molecular Medicine, AI Virtanen Institute, University of Kuopio, Kuopio, Finland

    K J Airenne, M O Hiltunen, M P Turunen, A-M Turunen & S Ylä-Herttuala

  2. Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland

    O H Laitinen & M S Kulomaa

  3. Department of Medicine, University of Kuopio, Kuopio, Finland

    S Ylä-Herttuala

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  1. K J Airenne
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Airenne, K., Hiltunen, M., Turunen, M. et al. Baculovirus-mediated periadventitial gene transfer to rabbit carotid artery. Gene Ther 7, 1499–1504 (2000). https://doi.org/10.1038/sj.gt.3301269

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