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  • Nonviral Transfer Technology
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Particle-mediated gene transfer into murine livers using a newly developed gene gun

Gene Therapy volume 7, pages 1132–1136 (2000)Cite this article

Abstract

Although particle-mediated gene transfer using gene gun technology has been applied for gene transfer into epidermis, applications of this technology to visceral tissues have not been well investigated. Although all helium gas-driven gene gun instruments have used macrocarriers to discharge DNA-coated microprojectiles so far, we used a newly developed gene gun instrument, in which a hammering bullet is used to discharge microprojectiles. With the gene gun, gold particles coated with lacZ expression plasmid were discharged to murine livers. LacZ expression was induced much more profoundly in the liver by particle-mediated gene transfer than by simple plasmid injection and electroporation-mediated gene transfer. LacZ expression was broadly and randomly distributed throughout the bombarded livers, indicating that particle-mediated gene transfer can induce transgene expression even at relatively distant areas from the surface of the bombarded tissue. Furthermore, although transgene expression was at its peak on day 2 after the bombardment, it was still detectable even on day 28. These results indicate that particle-mediated gene transfer with a newly developed gene gun may provide a new approach to gene therapy for human diseases.

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Acknowledgements

The authors are grateful to D Yong Mei, I Kijihana and A Kijihana of Nippon Medical & Chemical Instruments for technical assistance with the gene gun. This work was supported in part by a Grand-in-Aid for Scientific Research (B-10470140) from the Ministry of Education, Sciences, Sports and Culture of Japan.

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Authors and Affiliations

  1. Third Department of Internal Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara, 634-8522, Japan

    S Kuriyama, A Mitoro, H Tsujinoue, T Nakatani, H Yoshiji, T Tsujimoto, M Yamazaki & H Fukui

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  1. S Kuriyama
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Kuriyama, S., Mitoro, A., Tsujinoue, H. et al. Particle-mediated gene transfer into murine livers using a newly developed gene gun. Gene Ther 7, 1132–1136 (2000). https://doi.org/10.1038/sj.gt.3301192

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