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| June 1999, Volume 6, Number 6, Pages 1021-1029 |
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| Paper |
| Differential expression and secretion of alpha 1 anti-trypsin between direct DNA injection and implantation of transfected myoblast |
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| G Bou-Gharios1, D J Wells2, Q L Lu1, J E Morgan1 and T Partridge1 |
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1Muscle Cell Biology Group, MRC, Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Campus, London, UK
2Gene Targetting Group, Department of Neuromuscular Diseases, Division of Neurosciences and Psychological Medicine, Imperial College School of Medicine, Charing Cross Campus, London, UK
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Correspondence to: G Bou-Gharios, Muscle Cell Biology Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
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| Abstract |
| Muscle can be used for systemic delivery of non-muscle proteins. In order to investigate the relative effectiveness of direct DNA plasmid injection versus implantation of genetically modified myogenic cell lines, we have used the human alpha 1 anti-trypsin ( 1AT) cDNA driven by either cytomegalovirus (CMV) or the muscle creatine kinase 3.3 kb (MCK) promoter in immunodeficient mice. We dem- onstrate that the implantation of transfected myoblasts stably expressing the human 1AT cDNA generates a more persistent production of 1AT than does direct intramuscular injection of the same construct as plasmid DNA. Moreover, immunohistological labelling of muscle sections implanted with myoblasts show that the newly formed muscle fibres are those containing the human protein. |
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| Keywords |
| plasmid DNA; transfection; alpha 1 anti-trypsin; myoblast implantation |
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| Received 4 November 1998; accepted 15 February 1999 |
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| June 1999, Volume 6, Number 6, Pages 1021-1029 |
| Table of contents Previous Abstract Next Full text PDF |
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