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| August 1997, Volume 4, Number 8, Pages 773-782 |
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| Paper |
| Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery |
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| A Baker1, M Saltik1, H Lehrmann1, I Killisch1, V Mautner2, G Lamm1, G Christofori1 and M Cotten1 |
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1Institute for Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
2CRC Institute for Cancer Studies, The University of Birmingham, Edgbaston, UK
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| Abstract |
| A simple and inexpensive method of condensing and linking plasmid DNA to carrier adenovirus particles is described. The synthetic polycation polyethylenimine is used to condense plasmid DNA into positively charged 100 nm complexes. These PEI-DNA complexes are then bound to adenovirus particles through charge interactions with negative domains on the viral hexon. The resulting transfection complexes deliver plasmid DNA to cells by the adenovirus infectious route without interference from virus gene expression because psoralen-inactivated virus is employed. The PEI-DNA-adenovirus complexes display DNA delivery comparable to more sophisticated DNA virus complexes employing streptavidin/biotin linkage, but require no special reagents and are much easier to prepare. |
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| Keywords |
| transfection; synthetic polycations; DNA delivery; virus entry |
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| Received 8 January 1997; accepted 14 April 1997 |
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| August 1997, Volume 4, Number 8, Pages 773-782 |
| Table of contents Previous Abstract Next Article PDF |
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