FIGURE 4
FROM:
Targeting EGFR with metabolically biotinylated fiber-mosaic adenovirus
L Pereboeva, S Komarova, J Roth, S Ponnazhagan and D T Curiel
BACK TO ARTICLEFigure 4.
In vivo retargeting of mBfMAd to EGFR using animal model of ovarian carcinoma. CB17 SCID mice were injected i.p. with 10 
106 SKOV3ip1 cells to establish i.p. ovarian tumors. At day 21 after SKOV3ip injection, four groups of mice (n=7) were injected with Ad5luc or mBfMAd viruses either without adapter or preincubated with EGF-streptavidin. Viruses were injected i.p. at 1 1010 vp/animal. Mice were killed 48 h later, tumors and livers were excised and luciferase assay was performed in tissue lysates. Results are presented as luciferase expression (RLU) normalized for protein content (mg protein) for mBfMAd (a) and Ad5luc (b), and as tumor-to-liver ratio of luciferase expression (c). Tumor-to-liver ratio was calculated for individual animals, each represented as one dot on the graph. Bars represent averages of values in each group (n=7), P=0.01.
