1. ¹Max-Delbrück-Center for Molecular Medicine, Berlin, Germany
2. ²Institute of Biology, Humboldt-University of Berlin, Berlin, Germany
3. ³INSERM, U758, Ecole Normale Supérieure of Lyon, Lyon, France
4. ⁴Wohl Virion Center, Windeyer Institute of Medical Sciences, University College London, London, UK

Correspondence: Dr Wolfgang Uckert, Max-Delbrück-Center for Molecular Medicine, Robert-Rössle Str. 10, D13092 Berlin, Germany. E-mail: wuckert@mdc-berlin.de

Received 29 September 2006; Revised 22 November 2006; Accepted 27 November 2006; Published online 18 January 2007.

Abstract

The transfer of T-cell receptor (TCR) genes into primary human T-cells to endow their specificity toward virus-infected and tumor cells is becoming an interesting tool for immunotherapy. TCR-modified T cells are mainly generated by retrovirus-mediated gene transfer. To produce TCR-retrovirus particles, fibroblast packaging cell lines are the most common tool. We constructed two packaging cell lines based on the human suspension T-cell lymphoma line -Jurkat, which lacks endogenous TCR-chains and is therefore unable to express CD3 complexes on the cell surface. After supply of gag-pol (murine leukemia virus (Mo-MLV)) and env (GALV or MLV-10A1) genes, a green fluorescent protein (GFP)-encoding retrovirus vector was transduced into both packaging cell clones, which then stably produced GFP-retroviruses with titers of up to 4 10⁵ infectious particles (IP)/ml. After transfer of a TCR/-encoding retrovirus vector, -Jurkat/GALV and -Jurkat/10A1 cells expressed CD3 molecules on the cell surface. CD3-high expressing packaging cells were enriched by fluorescence-activated cell sorter sorting. In these cells, the CD3 expression level directly correlated with the titer of vector particles. TCR-retroviruses efficiently transduced human T-cell lines and primary T cells. In conclusion, the method allowed the fast and easy generation of high virus titer supernatants for TCR gene transfer.