Gene Therapy

FIGURE 1

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An enhanced autogene-based dual-promoter cytoplasmic expression system yields increased gene expression

J Finn, ACH Lee, I MacLachlan and P Cullis

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Figure 1.

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Plasmid diagrams of major constructs used. R023 is the basic autogene construct, containing the T7 RNAP gene driven by the T7, T3, and SP6 promoters (PTRI). L059 is the luciferase reporter gene cassette. R011 is the bicistronic autogene construct (R023+L059). L053 is the CMV-driven nuclear expression construct.

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