FIGURE 3
FROM:
Periocular injection of an adenoviral vector encoding pigment epithelium-derived factor inhibits choroidal neovascularization
P Gehlbach, A M Demetriades, S Yamamoto, T Deering, E J Duh, H S Yang, C Cingolani, H Lai, L Wei and P A Campochiaro
BACK TO ARTICLEFigure 3.
Recombinant PEDF protein diffuses into the eye from the periocular space. Adult C57BL/6 mice were given a periocular injection of 5 
l containing 3 g of recombinant PEDF in one eye and euthanized 2, 6, or 24 h after injection. Ocular frozen sections were immunohistochemically stained for PEDF. Red reaction product demonstrates high levels of PEDF in the orbit and sclera 2 (a), 6 (b), or 24 h (c) after injection. There is little staining for PEDF in the retina 2 or 6 h after injection, but there is unequivocal staining in the retina 24 h after injection (c). Incubation of primary antibody with PEDF eliminated all but faint background staining in the episclera (d). Staining for PEDF in control, uninjected eyes showed only faint background (e). A section from a Balb/C mouse given a periocular injection of 5 
109 particles of AdPEDF.11 showed red reaction product in the orbit and sclera and faint staining in the retina.
