Gene Therapy

FIGURE 2

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Periocular injection of an adenoviral vector encoding pigment epithelium-derived factor inhibits choroidal neovascularization

P Gehlbach, A M Demetriades, S Yamamoto, T Deering, E J Duh, H S Yang, C Cingolani, H Lai, L Wei and P A Campochiaro

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Figure 2.

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Levels of PEDF in ocular tissues after periocular injection of AdPEDF.11. Adult C57BL/6 mice were given a periocular injection of 5 times 109 particles of AdPEDF.11 on one side, and after 4 days the mice were killed and ocular and orbital tissues were dissected from both sides. Levels of PEDF were measured in tissue homogenates as described in Materials and Methods. The bars show the mean (plusminuss.e.m.) level of PEDF for the following number of experimental values for each tissue: serum, 4; orbital tissue, 8; optic nerve, 8; sclera, 8; choroid, 7; retina, 16; anterior segment, 8. Black bars show values on the side of the injection and gray bars show values from the contralateral side. For serum, the gray bars are values from uninjected mice. Statistical comparisons were made by a paired t-test.

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