FIGURE 5
FROM:
Water-soluble lipopolymer as an efficient carrier for gene delivery to myocardium
M Lee, J Rentz, S-O Han, D A Bull and S W Kim
BACK TO ARTICLEFigure 5.
Cytotoxicity of WSLP, PEI25 000, lipofectamine, SuperFect, or PEI1800 to A7R5 cells. A7R5 cells were seeded in 96-well microassay plates. Polymer/pCMV-Luc complexes were prepared as described in Materials and methods. Polymer/pCMV-Luc complexes were added to the cells and incubated for 4 h at 37°C. After the incubation, the transfection mixture was replaced with 100 
l of fresh DMEM medium supplemented with 10% FBS. The cells were incubated for an additional 20 h at 37°C. After the incubation, cell viability was measured by MTT assay. The data are expressed as mean values (
s.d.) of five experiments. *P<0.01 as compared with PEI25 000, lipofectamine, SuperFect, but no statistical significance as compared with PEI1800.
