Gene Therapy

FIGURE 12

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Exploiting hepatitis C virus activation of NFkappaB to deliver HCV-responsive expression of interferons alpha and big gamma

A A Matskevich and D S Strayer

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Figure 12.

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SV[HIVLTR](hIFNalpha) decreases detectable HCV transcripts. HepG2 cells were transduced with SV(BUGT), or with SV[wtHIVLTR]hIFNalpha at MOI=100, and 4 days later were transfected with HCV cDNA. One group was transfected with HCV cDNA and 24 h later treated with exogenous hIFNalpha at 50 pg/ml. Cells expressing HCV transcripts were visualized by in situ RT-PCR, incorporating fluorescent nucleotides into the HCV RT-PCR product, as described in Materials and methods. These data are representative of two independent experiments.

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