FIGURE 11

Paracrine IFN-induced biological responses in nontransduced cells. PRH (a) and HepG2 cells (b) were transduced with 100 MOI of SV[wtHIVLTR]mIFN, SV(BUGT), SV[muHIVLTR](mIFN), or mock-transduced. After 4 days, the cultures were transfected with HCV. In parallel, the same cell types were transfected with pISRE-Luc. (left panels) Supernatants from the transduced, HCV-transfected cell cultures were added to separate cultures of pISRE-Luc-transfected cells. Luciferase activity induced by IFNRE was measured on day 6 in a standard luminometric assay. (right panels) In coculture studies, pISRE-Luc-transfected cells were added to cultures of transduced, HCV-transfected cells. Luciferase activities were measured as indicated. Data represent means of independent experiments repeated a minimum of three times. Standard error bars are shown. Differences between SV[wtHIVLTR](IFN)+HCV and all other groups were significant at least at P<0.05 (a) and P<0.02 (b).