Gene Therapy

FIGURE 3

FROM:

CrmA gene expression protects mice against concanavalin-A-induced hepatitis by inhibiting IL-18 secretion and hepatocyte apoptosis

M Fujino, M Kawasaki, N Funeshima, Y Kitazawa, M Kosuga, K Okabe, M Hashimoto, H Yaginuma, K Mikoshiba, T Okuyama, S Suzuki and X-K Li

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Figure 3.

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CrmA protected against ConA-induced hepatocyte apoptosis in mice. (a) DNA fragmentation was demonstrated by the TUNEL method. At 18 h after injection of ConA, we observed a large number of TUNEL-positive cells in the CrmA-negative liver (CrmA(-)), but not in the CrmA-expressed liver (CrmA(+)). (b) The extent of the hepatocyte apoptosis was semiquantitatively measured by LM-PCR assay. The apparent changes of internucleosomal DNA cleavage were confirmed after ConA injection, and were absent after CrmA expression. (c) Activated caspase-3 form p17 was confirmed by identifying a cleaved caspase-3 antibody. CrmA (-), 109 PFU of AxCALNLCrmA only; CrmA (+), 109 PFU of AxCALNLCrmA and 109 PFU of AxCANCre. The data are representative of three separate experiments.

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