Figure 4 - Secreted phospholipase A2 (sPLA2) inhibitors and [3H]AA release and LES tone.

From the following article

Signal transduction in lower esophageal sphincter circular muscle

Piero Biancani and Karen M. Harnett

GI Motility online (2006)



a: LES muscle was incubated with [3H] arachidonic acid (3 muCi/mL), and then washed to remove unincorporated [3H]AA, and incubated in Krebs alone (control), AM5 or MJ33 (10-4 M). [3H]AA release, an index of spontaneous PLA2 activity, was significantly reduced by the nonselective sPLA2 inhibitor AM5, and by the selective group I PLA2 inhibitor MJ33. B: In LES muscle strips the group I PLA2 inhibitor MJ33 decreased LES tone, and tetrodotoxin (10-4 M) had no effect on the decrease. The cytosolic PLA2 inhibitor AACOCF3 had no effect excluding involvement of cytosolic PLA2. These data suggest that group I sPLA2 may play a role in the maintenance of LES tone and that this PLA2 may not come from nerve terminals. Values are means + SE of three to six animals.

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