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Interleukin-10 promoter single-nucleotide polymorphisms as markers for disease susceptibility and disease severity in leprosy

Genes & Immunity volume 5, pages 592–595 (2004)Cite this article

Abstract

We have determined IL-10 promoter genotypes of five single-nucleotide polymorphisms (SNPs): T−3575A, A−2849G, C−2763A, -A−1082G and C−819T. The haplotype frequencies were defined in healthy subjects compared to leprosy patients, and analyzed for their occurrence in multi- (MB) vs paucibacillary (PB) as severe and mild forms of leprosy, respectively. Haplotypes defined by three SNP positions (−3575, −2849 and −2763) captured significant differences between controls and patients (P=0.04). The haplotype carrying −3575A, −2849G and −2763C was associated with resistance to leprosy and to the development of severe forms of the disease using either a binomial (controls vs cases, P=0.005, OR=0.35, CI=0.13–0.91) or ordinal (controls vs PB vs MB, P=0.006, OR=0.32, CI=0.12–0.83) model. By contrast, the IL-10 haplotype −3575T/−2849A/−2763C was found to be associated with susceptibility to leprosy per se (P=0.027, OR=2.37, CI=1.04–5.39), but not leprosy type. The data suggest that the IL-10 locus contributes to the outcome of leprosy.

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Acknowledgements

We are grateful to Valcemir F Silva-Filho for technical assistance and Denise Vieira for sample collection. These studies were supported in part by the World Health Organization/World Bank/TDR, the Netherlands Leprosy Foundation (NLR) and the Royal Netherlands Academy of Arts and Sciences (KNAW).

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Authors and Affiliations

  1. Department of Tropical Medicine, Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, RJ, Brazil

    MO Moraes, PR Vanderborght, JAC Nery, AR Santos, EP Sampaio & EN Sarno

  2. Departamento de Epidemiologia e Métodos Quantitativos em Saúde (DEMQS), Escola Nacional de Saúde Pública (ENSP), FIOCRUZ, Rio de Janeiro, Brazil

    AG Pacheco

  3. Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands

    JJM Schonkeren & TWJ Huizinga

  4. Laboratório de Imunogénetica, Instituto Nacional do Cancer, Ministério da Saúde, Rio de Janeiro, RJ, Brazil

    ME Moraes & JR Moraes

  5. Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands

    THM Ottenhoff

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  1. MO Moraes
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  2. AG Pacheco
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Correspondence to MO Moraes.

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Moraes, M., Pacheco, A., Schonkeren, J. et al. Interleukin-10 promoter single-nucleotide polymorphisms as markers for disease susceptibility and disease severity in leprosy. Genes Immun 5, 592–595 (2004). https://doi.org/10.1038/sj.gene.6364122

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