1. ¹Functional Biology Department, University of Oviedo, Spain
2. ²Department of Immunology, Hospital Central de Asturias, Oviedo, Spain

Correspondence: C López-Larrea, Department of Immunology, E-33006, Oviedo Spain. E-mail: inmuno@hca.es

Abstract

To study the transcriptional regulation of the human complement component C7, a 1 kb promoter fragment was cloned and the transcription start site was determined. C7 is expressed by the hepatoma-derived cell line Hep-3B, but not by Hep-G2. Transfection of these cell lines with different C7 promoter–luciferase constructs demonstrated that 1 kb of the 5'-flanking region contains the necessary elements for driving C7 transcription in a tissue-specific manner and showed that the sequence between -29/+102 retained the majority of C7 promoter activity in Hep-3B. Electrophoretic mobility shift assays suggested that the binding of the C/EBP transcription factor to a C/EBP sequence located at +42 is essential for C7 expression. To investigate whether the absence of C/EBP expression in Hep-G2 cells is responsible for the lack of C7 transcription, Hep-G2 cells were transfected with a C/EBP expression vector. C/EBP transactivated the C7 luciferase reported gene and restored the C7 expression in Hep-G2 cells.