Laboratory Study
Eye advance online publication 20 February 2009; doi: 10.1038/eye.2009.29
Comparison of in vitro susceptibilities of Gram-positive cocci isolated from ocular infections against the second and fourth generation quinolones at a tertiary eye care centre in South India
This work was presented orally in the 42 annual meeting of Ocular Microbiology and Immunology Group, held on 7th November 2008 at Atlanta, USA
A K Reddy1, P Garg2, M R Alam3, U Gopinathan1, S Sharma1 and S Krishnaiah4
- 1Jhaveri Microbiology Centre, Hyderabad Eye Research Foundation, LV Prasad Eye Institute, Hyderabad, India
- 2Cornea and Anterior Segment services, LV Prasad Eye Institute, Hyderabad, India
- 3Cornea and Anterior Segment Services, MAI Eye Institute and Hospital, Farmgate, Dhaka, Bangladesh
- 4Internatoional centre for Advancement of Rural Eye Care, LV Prasad Eye Institute, Hyderabad, India
Correspondence: AK Reddy, Jhaveri Microbiology Centre, Prof Brien A Holden Eye Research Centre, Hyderabad Eye Research Foundation, LV Prasad Eye Institute, Banjara Hills, Hyderabad, AP 500034, India. Tel: +91 40 30612517; Fax: +91 40 23548271; E-mail: ashokkumar@lvpei.org
Received 12 December 2008; Revised 20 January 2009; Accepted 21 January 2009; Published online 20 February 2009.
Abstract
Purpose
To compare the in vitroantimicrobial susceptibilities of Gram-positive cocci isolated from the ocular infections to the second and fourth generation fluoroquinolones at a tertiary eye care centre in south India.
Methods
A retrospective review of microbiology records at LV Prasad eye institute, Hyderabad, India, identified 787 Gram-positive cocci isolated from different ocular infections between January 2005 to May 2008.The isolates were identified using culture characteristics and biochemical tests. In vitroantibiotic susceptibility of the isolates was determined by using Kirby|[ndash]|Bauer disc diffusion method. We analysed the susceptibility data of ciprofloxacin, ofloxacin, gatifloxacin, and moxifloxacin.
Results
Out of 787 isolates, 147 (18.7|[percnt]|) were Staphylococcus aureus, 279 (35.2|[percnt]|) were coagulase-negative Staphylococci, 357 (45.4|[percnt]|) were Streptococcus pneumoniae,and 4 (0.4|[percnt]|) were other Streptococcus species. Of the four quinolones, susceptibility to gatifloxacin was highest (85.6|[percnt]|) followed by ofloxacin (65.6|[percnt]|), moxifloxacin (63.9|[percnt]|), and ciprofloxacin (60.5|[percnt]|). In all, 33 (4.2|[percnt]|) of 787 isolates were resistant to all the four fluoroquinoles. S. aureusand coagulase-negative Staphylococcus isolates that were resistant to ciprofloxacin and ofloxacin were most susceptible to gatifloxacin. S. pneumoniaewere more susceptible to gatifloxacin, ofloxacin, and ciprofloxacin than moxifloxacin.
Conclusions
In our institute, we observed that gatifloxacin is more potent than moxifloxacin against Gram-positive cocci isolated from ocular infections.
Keywords:
Staphylococcus aureus, coagulase-negative Staphylococcus, ciprofloxacin, gatifloxacin, moxifloxacin
