Laboratory Study
Eye (2008) 22, 439–444; doi:10.1038/sj.eye.6702735; published online 16 February 2007
Expression of matrix metalloproteinases of human lens epithelial cells in the cultured lens capsule bag
J H Li1, N L Wang1 and J J Wang2
- 1Beijing Tongren Eye Center, Beijing Tongren Hospital, Affiliated Hospital of Capital Medical University, Beijing, China
- 2Beijing Institute of Ophthalmology, Beijing, China
Correspondence: NL Wang, Beijing Tongren Eye Center, Beijing Tongren Hospital, Affiliated Hospital of Capital Medical University, No. 1 Dongjiaomingxiang, Dongchen district, Beijing, 100730, PR China. Tel: + 86 010 58269920; Fax: + 86 010 58269920; E-mail: wningli@trhos.com
Received 30 June 2006; Revised 28 December 2006; Accepted 28 December 2006; Published online 16 February 2007.
Abstract
Aim of purpose
To observe the different expression of matrix metalloproteinases (MMPs) between pre- and postoperation of sham cataract surgery in vitrohuman lens capsule bag model from the same donor eye in order to investigate a possible role of MMPs in posterior capsule opacification (PCO).
Methods
Sham cataract surgeries were performed in six human donor eyes. Immunohistochemical staining was used to detect the expression of MMP-2 and -9 of human lens epithelial cells (LECs) on the anterior capsulorhexis. LEC migration on posterior capsule of human lens from the same donor eye was observed in a modified capsule bag model without pin. Total MMP-2 and -9 protein production were determined by enzyme-linked immunosorbent assay at days 2, 10, 20, and 30 postoperation, respectively.
Results
MMP-2 and -9 could not be detected immunohistochemically on the anterior capsulorhexis preoperation of cataract. Lens epithelia cells at the equator began to migrate by day 4. A confluent monolayer of lens epithelia cells was present on the posterior capsule at day 20. Total MMP-2 and -9 protein production increased with time with maximum levels reached on day 30.
Conclusion
MMP-2 and -9 were showed to be upregulated following sham cataract surgery. MMP expression could play an important role in PCO.
Keywords:
lens epithelial cells, matrix metalloproteinases, posterior capsule opacification
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