Laboratory Study
Eye (2008) 22, 1508–1513; doi:10.1038/eye.2008.92; published online 28 March 2008
Irrigation with thapsigargin and various concentrations of 5-fluorouracil in a sealed-capsule irrigation device in young rabbit eyes to prevent after-cataract
Presented at the XXV Congress of the ESCRS on 10 September, 2007, in Stockholm, Sweden.
None of the authors has a proprietary or financial interest in any material, method, or product mentioned.
M T Abdelwahab1, M Kugelberg1 and C Zetterström2
- 1St Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
- 2Ullevål University Hospital, University of Oslo, Oslo, Norway
Correspondence: M Kugelberg, St Erik's Eye Hospital, SE-112 82 Stockholm, Sweden Tel: +46 8 672 30 00; Fax: +46 8 651 07 85; E-mail: maria.kugelberg@ sankterik.se
Received 24 October 2007; Revised 26 February 2008; Accepted 26 February 2008; Published online 28 March 2008.
Abstract
Aim
To investigate the effect on after-cataract and synechiae formation after clear lens extraction and irrigation with different substances in a sealed-capsule irrigation device.
Setting
St Erik's Eye Hospital, Stockholm, Sweden.
Methods
Clear lens extraction was performed in one eye of 42 4-week-old rabbits. The sealed-capsule irrigation device was applied and the sealed system was irrigated for 2 min with 20 ml of one of four substances: balanced salt solution (BSS), thapsigargin, 5-fluorouracil (5-FU) 50 mg/ml, or 5-FU 25 mg/ml. The substance then was washed out for 10 s with BSS. The eyes were left aphakic. Formation of after-cataract and synechiae was evaluated during two clinical examinations 3.5 and 5.5 weeks postoperatively, by photographs 5 weeks postoperatively, and by histologic evaluation of haematoxylin–eosin-stained slides after the 6-week end point postoperatively. After-cataract and synechiae were graded on a scale from 0 to 4. Kruskal–Wallis analysis of variance with multiple comparisons was used for statistical analyses.
Results
5-FU 50 mg/ml prevented after-cataract and synechiae formation best when compared with all other substances at all evaluations. 5-FU 25 mg/ml was not as effective, and thapsigargin was ineffective in this animal model.
Conclusion
5-FU 50 mg/ml used in the sealed-capsule irrigation device satisfactorily prevents after-cataract and synechiae. Thapsigargin was ineffective in this animal model.
Keywords:
sealed-capsule irrigation, perfect capsule, fluorouracil, thapsigargin, paediatric cataract surgery, posterior capsule opacification
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