Laboratory Study
Eye (2008) 22, 128–131; doi:10.1038/sj.eye.6702751; published online 9 February 2007
Protein carbonyl levels in the aqueous humour and serum of patients with pseudoexfoliation syndrome
There are no any proprietary interests or research funding
R Ya
c
1, I Ersöz2, M Erdurmu
1, A Gürel3 and S Duman2
- 1Department of Ophthalmology, Medical School, Fatih University, Ankara, Turkey
- 2Ankara Training and Research Hospital, Department of Ophthalmology, Ankara, Turkey
- 3Department of Biochemistry and Clinical Biochemistry, Medical School, Zonguldak Karaelmas University, Zonguldak, Turkey
Correspondence: R Yagc, Fakülteler M Keskin S 9/3 Cebeci, 06590 Çankaya/Ankara, Turkey. Tel: +90 312 319 90 10; Fax: +90 312 221 32 76; E-mail: ramazanyagci@yahoo.com
Received 3 September 2006; Accepted 4 January 2007; Published online 9 February 2007.
Abstract
Purpose
Protein oxidation is an oxidative stress marker and the oxidation of proteins is analysed by measuring the carbonyl groups. Protein oxidation can have a role in the physiopathology of pseudoexfoliation (PEX) syndrome. The aim of this study was to investigate the protein oxidation in the aqueous humour and serum of cataract patients with and without PEX.
Methods
A multicenter study was carried out. Aqueous humour and serum samples were collected from patients who underwent routine cataract surgery. Patients were divided into PEX (n=29) and control (n=27) groups. Patients had no elevated intraocular pressure or glaucoma. Spectrophotometer was used to measure protein carbonyl (PC) levels in the samples.
Results
Mean PC concentration in the PEX aqueous (2.18
1.51 nmol/l) and serum (119.6213.2 nmol/l) samples was significantly higher than that measured in the control aqueous (1.310.47 nmol/l) and serum (105.8511.76 nmol/l) samples, respectively (P< 0.001).
Conclusion
The increased PC levels in the aqueous humour and serum of PEX patients suggest that protein oxidation may play a role in the physiopathology of PEX.
Keywords:
protein carbonyl, aqueous humour, oxidative stress, protein oxidation, pseudoexfoliation
