Laboratory Study
Eye (2007) 21, 658–664. doi:10.1038/sj.eye.6702353; published online 21 April 2006
Decrease in reducing power of aqueous humor originating from glaucomatous rabbits
E Beit-Yannai1, V Trembovler2 and A S Solomon3
- 1Department of Clinical Pharmacology, Ben-Gurion University of the Negev, Beer-Sheva, Israel
- 2Pharmacology Department, The Hebrew University Jerusalem, Jerusalem, Israel
- 3Goldschleger Eye Research Institute, Tel-Aviv University, Tel-Aviv, Israel
Correspondence: E Beit-Yannai, Department of Clinical Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, POB 653, Beer-Sheva 84105, Israel. Tel: +972 8 647 7374; Fax: +972 8 647 7629; E-mail: bye@bgu.ac.il
Received 29 August 2005; Accepted 7 March 2006; Published online 21 April 2006.
Abstract
Aim
To evaluate changes in the reducing power of aqueous humor (AH) with cyclic voltammetry (CV) and HPLC-EC.
Methods
NZW Rabbits exhibiting a sporadic mutation causing bilaterally buphthalmus eyes were set for intra ocular pressure (IOP) and eye size measurements. AH was obtained under anesthesia, from congenital glaucomatic rabbits (CGR, n=6) and age-matched controls (CON; n=6). The AH samples were analysed by CV and HPLC-EC.
Results
CGR IOP was found to be significantly higher than in CON (33.5
1.1 and 14.21.0 mmHg, respectively), eye size was 18.25 and 13.9 cm, respectively. CV analysis revealed two anodic currents representing two groups of low molecular weight antioxidant (LMWA). The two anodic potentials were equal for the two tested groups, indicating the same components of LMWA. The first anodic current of CGR was only 30% of the CON rabbits (2.11 vs7.17 
A/mg protein, t-test: P<0.05). As the main hydrophilic components of the first anodic current are known to be uric acid (UA) and ascorbic acid (AA), they were analysed for exact content by HPLC-EC. UA and AA levels were significantly lower in the CGR group (UA: 17.13.2 and 189.175.70 M/mg, AA: 1.10.3 and 4.82.0 M/mg protein respectively).
Conclusions
Changes in the reducing power, as indicated by CV analysis, of CGR AH, is probably a result of chronic oxidative stress caused by the pathology. The differences in the first anodic wave are mainly due to a fall in the concentration of UA and AA.
Keywords:
glaucoma, aqueous humor, cyclic voltammetry, rabbit, antioxidants, intra ocular pressure
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