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EMBO reports AOP
doi:10.1038/embor.2008.55
Published online: 2 May 2008
Switching of chromatin-remodelling complexes for oestrogen receptor-
Maiko Okada1, Shin-ichiro Takezawa1, 2, Yoshihiro Mezaki1, Ikuko Yamaoka1, 2, Ichiro Takada1, Hirochika Kitagawa1 & Shigeaki Kato1, 2
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1 The Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
2 ERATO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan
To whom correspondence should be addressed
Shigeaki Kato Tel: +81 3 5841 8478; Fax: +81 3 5841 8477; E-mail: uskato@mail.ecc.u-tokyo.ac.jp
Received 13 September 2007; Accepted 11 March 2008; Published online 2 May 2008.
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Abstract
The female sex steroid hormone oestrogen stimulates both cell proliferation and cell differentiation in target tissues. These biological actions are mediated primarily through nuclear oestrogen receptors (ERs). The ligand-dependent transactivation of ERs requires several nuclear co-regulator complexes; however, the cell-cycle-dependent associations of these complexes are poorly understood. By using a synchronization system, we found that the transactivation function of ER at G2/M was lowered. Biochemical approaches showed that ER associated with two discrete classes of ATP-dependent chromatin-remodelling complex in a cell-cycle-dependent manner. The components of the NuRD-type complex were identified as G2/M-phase-specific ER co-repressors. Thus, our results indicate that the transactivation function of ER is cell-cycle dependent and is coupled with a cell-cycle-dependent association of chromatin-remodelling complexes.
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