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EMBO reports 9, 8, 781–787 (2008)
doi:10.1038/embor.2008.112
AOP Published online: 20 June 2008
Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA
Tung-Gia Du1, 2*, Stephan Jellbauer1, 2*, Marisa Müller1, 2, 3, Maria Schmid1, 2, Dierk Niessing1, 2, 3 & Ralf-Peter Jansen1, 2
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1 Gene Center, Ludwig-Maximilians-Universität München, Feodor-Lynen-Strasse 25, D-81377 Munich, Germany
2 Munich Center for Integrated Protein Science (CiPSM), Ludwig-Maximilians-Universität München, Feodor-Lynen-Strasse 25, D-81377 Munich, Germany
3 Institute of Structural Biology, Helmholtz Zentrum München, D-81377 Munich, Germany
To whom correspondence should be addressed
Ralf-Peter Jansen Tel: +49 (0)89 2180 76903; Fax: +49 (0)89 2180 76948; E-mail: rjansen@lmb.uni-muenchen.de
* These authors contributed equally to this work
Received 23 November 2007; Accepted 9 May 2008; Published online 20 June 2008.
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Abstract
Cytoplasmic localization and localized translation of messenger RNAs contribute to asymmetrical protein distribution. Recognition of localized mRNAs by RNA-binding proteins can occur in the cytoplasm or, alternatively, co- or post-transcriptionally in the nucleus. In budding yeast, mRNAs destined for localization are bound by the She2 protein before their nuclear export. Here, we show that a specific transcript, known as ASH1 mRNA, and She2 localize specifically to the nucleolus when their nuclear export is blocked. Nucleolar She2 localization is enhanced in a She2 mutant that cannot bind to RNA. A fusion protein of the amino terminus of She3 and She2 (She3N-She2) fails to enter the nucleus, but does not impair ASH1 mRNA localization. Instead, these cells fail to distribute Ash1 protein asymmetrically, which is caused by a defective translational control of ASH1 mRNA. Our results indicate that the nucleolar transit of RNA-binding proteins such as She2 is necessary for the correct assembly of translationally silenced localizing messenger ribonucleoproteins.
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