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EMBO reports 9, 3, 267–272 (2008)
doi:10.1038/embor.2008.1
AOP Published online: 1 February 2008
Regulation of an inducible promoter by an HP1 –HP1 switch
Bogdan Mateescu1, 2, Brigitte Bourachot1, 2, Christophe Rachez1, 2, Vasily Ogryzko3 & Christian Muchardt1, 2
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1 Institut Pasteur, Département de Biologie du Développement, URA2578 du CNRS, cedex 15, France
2 Unité de Régulation Epigénétique, INSERM Avenir, Institut Pasteur, 25 rue du docteur Roux, 75724 Paris cedex 15, France
3 Unité Interactions Moléculaires et Cancer, Institut Gustave Roussy, UMR8126 du CNRS, 39 rue Camille Desmoulins, 94805 cedex Villejuif, France
To whom correspondence should be addressed
Christian Muchardt Tel: +33 1 45 68 85 25; Fax: +33 1 40 61 30 33; E-mail: muchardt@pasteur.fr
Received 24 July 2007; Accepted 19 December 2007; Published online 1 February 2008.
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Abstract
The mammalian heterochromatin protein 1 (HP1) family of proteins was recently shown to be involved in transient repression of inducible promoters. One of these promoters is the HIV1 long terminal repeat, which, during viral latency, recruits a non-processive RNA polymerase II (RNAPII) that synthesizes a short regulatory transcript. Here, we have used this promoter to examine the interplay of HP1 , HP1 and HP1 with RNAPII. We find that, in the absence of stimulation, HP1 is present on the promoter together with the non-processive RNAPII and functions as a negative regulator. On activation, HP1 bound to methylated H3K9 is rapidly released concurrent with histone H3 phospho-acetylation, and is replaced by HP1. This isoform localizes to the promoter but also inside the coding region, together with the processive RNAPII. Our data show that HP1 recruitment–release is a sequential mechanism that is precisely regulated and highly dependent on transcription.
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