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EMBO reports 8, 8, 749–755 (2007)
doi:10.1038/sj.embor.7401025
AOP Published online: 6 July 2007
Subunits act independently in a cyclic nucleotide-activated K+ channel
Abhishek Cukkemane1*, Bärbel Grüter1*, Kerstin Novak1, Thomas Gensch1, Wolfgang Bönigk1, Tanja Gerharz2, U Benjamin Kaupp1 & Reinhard Seifert1
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1 Institut für Neurowissenschaften und Biophysik INB-1, Abteilung Zelluläre Biophysik and Forschungszentrum Jülich, Leo-Brandt-Strasse, 52425 Jülich, Germany
2 Institut für Biotechnologie IBT-1, Forschungszentrum Jülich, Leo-Brandt-Strasse, 52425 Jülich, Germany
To whom correspondence should be addressed
Reinhard Seifert Tel: +49 2461618066; Fax: +49 2461614216; E-mail: r.seifert@fz-juelich.de
* These authors contributed equally to this work
Received 16 February 2007; Accepted 28 May 2007; Published online 6 July 2007.
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Abstract
Ion channels gated by cyclic nucleotides have crucial roles in neuronal excitability and signal transduction of sensory neurons. Here, we studied ligand binding of a cyclic nucleotide-activated K+ channel from Mesorhizobium loti and its isolated cyclic nucleotide-binding domain. The channel and the binding domain alone bind cyclic AMP with similar affinity in a non-cooperative manner. The cAMP sensitivities of binding and activation coincide. Thus, each subunit in the tetrameric channel acts independently of the others. The binding and gating properties of the bacterial channel are distinctively different from those of eukaryotic cyclic nucleotide-gated channels.
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