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8, 2, 173–180 (2007)
doi:10.1038/sj.embor.7400877
Figures and Tables
Mammalian SGO2 appears at the inner centromere domain and redistributes depending on tension across centromeres during meiosis II and mitosis
Rocío Gómez, Ana Valdeolmillos, María Teresa Parra, Alberto Viera, Candelas Carreiro, Fernando Roncal, Julio S Rufas, José L Barbero & José A Suja
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Figure 1
SGO2 distribution during meiosis I. Double immunolabellings of SGO2 (green) with kinetochores (ACA; red; columns 1–3); or RAD21 (red; column 4); and counterstaining with 4,6-diamidino-2-phenylindole (DAPI; blue; column 3). (A–D) Late diplotene; (E–H) diakinesis; (I–L) metaphase I; (M–P) anaphase I; and (Q–T) telophase I spermatocytes. All images are projections of different focal planes throughout the cell volume. ACA, anti-centromere autoantibody.
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Figure 2
SGO2 and RAD21, but not REC8, show a complex arrangement at the inner domain of metaphase I centromeres and change their relative distributions during telophase I. Double immunolabellings of SGO2 (green) with kinetochores (ACA; pseudocoloured in pink; A–E'), RAD21 (red; F–J') or REC8 (blue; K–O') in metaphase I (A–C',F–H',K–M') and telophase I (D–E',I–J',N–O') centromeres. (A–A",F–F",K–K") Side views of autosomal metaphase I bivalents. (B,B',G,G',L,L') Side views of metaphase I centromeres. (C,C',H,H',M,M') Top views of metaphase I centromeres. (D,D',I,I',N,N') Side views and (E,E',J,J',O,O') top views of telophase I centromeres. ACA, anti-centromere autoantibody.
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Figure 3
SGO2 distribution during meiosis II. Double immunolabellings of SGO2 (green) with kinetochores (ACA; red; columns 1–3), or RAD21 (red; column 4), and counterstaining with 4,6-diamidino-2-phenylindole (DAPI; blue; column 3). (A–D) Early interkinesis; (E–H) late interkinesis; (I–L) prophase II; (M–P) metaphase II; (Q–T) anaphase II; and (U–X) telophase II spermatocytes. Arrowheads in (M,N) indicate an SGO2 band at one centromere. All images are projections of different focal planes throughout the cell volume. ACA, anti-centromere autoantibody.
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Figure 4
SGO2 redistributes within the centromeres throughout chromosome congression to the metaphase II plate depending on microtubule attachment to sister kinetochores. Double immunolabellings of SGO2 (green) with kinetochores (ACA; pseudocoloured in pink; A–F'), BubR1 (red; G–I') or MCAK (blue; J–L') in prometaphase II/metaphase II (A–D',G–L'), anaphase II (E,E') and telophase II (F,F') enlarged centromeres. (A-C',G-I',J-L') Side views of enlarged prometaphase II/metaphase II centromeres. (D,D') Top views of metaphase II and (E,E') anaphase II centromeres. (F,F') Top view of a telophase II centromere. (M) Partial view of a prometaphase II spermatocyte labelled for SGO2 (green), kinetochores (ACA; pseudocoloured in pink) and microtubules (tubulin; pseudocoloured in pink). (N,O) Two selected centromeres from colchicine-treated prometaphase II spermatocytes labelled for SGO2 (green) and kinetochores (ACA; pseudocoloured in pink). ACA, anti-centromere autoantibody; MCAK, mitotic centromere-associated kinesin.
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