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EMBO reports 8, 12, 1162–1169 (2007)
doi:10.1038/sj.embor.7401097
AOP Published online: 2 November 2007
Src kinase modulates the activation, transport and signalling dynamics of fibroblast growth factor receptors
Emma Sandilands1, Shiva Akbarzadeh2, Anna Vecchione2, David G McEwan1, Margaret C Frame1 & John K Heath2
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1 Cancer Research UK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Glasgow G61 1BD, UK
2 Cancer Research UK Growth Factor Group, School of Biosciences, University of Birmingham, Edgbaston, Birmingham B125 2TT, UK
To whom correspondence should be addressed
John K Heath Tel: +44 (0) 121 414 7533; Fax: +44 (0) 121 414 5925; E-mail: j.k.heath@bhm.ac.uk
Received 16 May 2007; Accepted 19 September 2007; Published online 2 November 2007.
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Abstract
The non-receptor tyrosine kinase Src is recruited to activated fibroblast growth factor receptor (FGFR) complexes through the adaptor protein factor receptor substrate 2 (FRS2). Here, we show that Src kinase activity has a crucial role in the regulation of FGFR1 signalling dynamics. Following receptor activation by ligand binding, activated Src is colocalized with activated FGFR1 at the plasma membrane. This localization requires both active Src and FGFR1 kinases, which are inter-dependent. Internalization of activated FGFR1 is associated with release from complexes containing activated Src. Src-mediated transport and subsequent activation of FGFR1 require both RhoB endosomes and an intact actin cytoskeleton. Chemical and genetic inhibition studies showed strikingly different requirements for Src family kinases in FGFR1-mediated signalling; activation of the phosphoinositide-3 kinase–Akt pathway is severely attenuated, whereas activation of the extracellular signal-regulated kinase pathway is delayed in its initial phase and fails to attenuate.
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