|
 |
|
|
|
EMBO reports 7, 9, 874–879 (2006)
doi:10.1038/sj.embor.7400780
Organellar proteomics: turning inventories into insights
Jens S Andersen1 & Matthias Mann2
|
|
|
|
1 Centre for Experimental Bioinformatics (CEBI), University of Southern Denmark, Campusvej 55, DK-5230 Odense, Denmark
Tel: +45 6550 2365; Fax: +45 6593 3018
e-mail: jens.andersen@bmb.sdu.dk
2 Department of Proteomics and Signal Transduction, Max-Planck Institute for Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany
Tel: +49 89 8578 2557; Fax: +49 89 8578 2219;
e-mail: mmann@biochem.mpg.de
Received 8 May 2006; Accepted 7 July 2006.
|
|
|
|
Abstract
Subcellular organization is yielding to large-scale analysis. Researchers are now applying robust mass-spectrometry-based proteomics methods to obtain an inventory of biochemically isolated organelles that contain hundreds of proteins. High-resolution methods allow accurate protein identification, and novel algorithms can distinguish genuine from co-purifying components. Organellar proteomes have been analysed by bioinformatic methods and integrated with other large-scale data sets. The dynamics of organelles can also be studied by quantitative proteomics, which offers powerful methods that are complementary to fluorescence-based microscopy. Here, we review the emerging trends in this rapidly expanding area and discuss the role of organellar proteomics in the context of functional genomics and systems biology.
|
 |
|
Top of page MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated | |
|
top  |
|
|
|
