close window   7, 12, 1206–1210 (2006) doi:10.1038/sj.embor.7400834 Figures and Tables Chromatin at the crossroads. Meeting on Signalling to Chromatin Epigenetics Adam G West & Haico van Attikum   Figures     Figure 1 Model of MDC1-regulated phosphorylation of histone H2AX. (1) The MRE11, RAD50 and NBS1 (MRN) complex binds DNA ends at sites of DNA damage and recruits ataxia telangiectasia mutated (ATM), which phosphorylates proximal H2AX. (2,3) Mediator of DNA damage checkpoint 1 (MDC1) binds phosphorylated proximal H2AX and recruits more MRN–ATM. The new pool of ATM phosphorylates more distal H2AX. These events could contribute to the 'spreading' of H2AX phosphorylation to more distal chromatin regions. Figure 2 Spatial organization of cytokine loci. Schematic representation of the murine Th2 (Il3/Rad50/Il13/Il4), interferon- (Ifn) and Th1 (Lt/Tnf/Lt) loci used in 3C chromosome-interaction analyses by Flavell and co-workers (Spilianakis et al, 2005). Vertical arrows on the top represent the DNaseI-hypersensitive sites so far characterized at the Th2 locus. Figure 3 Interchromosomal interaction between the interferon- and Th2 loci revealed by fluorescence in situ hybridization. CD4+ naive T cells were hybridized with a SpectrumGreen-dUTP-labelled probe for the interferon- (Ifn) locus and a rhodamine–dUTP-labelled probe for the Th2 locus. 4',6-diamidino-2-phenylindole (DAPI) staining of nuclei shows the presence of DNA. Figure courtesy of R. Flavell and co-workers (Spilianakis et al, 2005).
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