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EMBO reports 7, 12, 1259–1265 (2006)
doi:10.1038/sj.embor.7400824
AOP Published online: 10 November 2006
The Survivin–Crm1 interaction is essential for chromosomal passenger complex localization and function
Shirley K Knauer, Carolin Bier, Negusse Habtemichael & Roland H Stauber
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Georg-Speyer-Haus, Paul-Ehrlich-Strasse 42–44, Frankfurt D-60596, Germany
To whom correspondence should be addressed
Roland H Stauber
† Present address: Department of Otorhinolaryngology, Molecular and Cellular Oncology, University Hospital of Mainz, Langenbeckstrasse 1, 55101 Mainz, Germany
Received 6 June 2006; Accepted 4 September 2006; Published online 10 November 2006.
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Abstract
The chromosomal passenger complex (CPC) of Aurora-B, Borealin, INCENP (inner centromere protein) and Survivin coordinates essential chromosomal and cytoskeletal events during mitosis. Here, we show that the nuclear export receptor Crm1 is crucially involved in tethering the CPC to the centromere by interacting with a leucine-rich nuclear export signal (NES), evolutionarily conserved in all mammalian Survivin proteins. We show that inhibition of the Survivin–Crm1 interaction by treatment with leptomycin B or by RNA-interference-mediated Crm1 depletion prevents centromeric targeting of Survivin. The genetic inactivation of the Survivin–Crm1 interaction by mutation of the NES affects the correct localization and function of Survivin and the CPC during mitosis. By contrast, CPC assembly does not seem to require the Survivin–Crm1 interaction. Our report shows the functional significance of the Survivin–Crm1 interface and provides a novel link between the mitotic effector Crm1 and the CPC.
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