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EMBO reports 7, 11, 1154–1161 (2006)
doi:10.1038/sj.embor.7400801
AOP Published online: 29 September 2006
Live imaging of neural structure and function by fibred fluorescence microscopy
Pierre Vincent1, 2, 6, Uwe Maskos3, 6, Igor Charvet4, 6, Laurence Bourgeais1, 2, Luc Stoppini5, Nathalie Leresche1, 2, Jean-Pierre Changeux3, Régis Lambert1, 2, Paolo Meda4 & Danièle Paupardin-Tritsch1, 2
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1 Université Pierre et Marie Curie-Paris, ERT 1059, Boîte Courrier number 16, 9 Quai St Bernard, 75005 Paris, France
2 CNRS, UMR 7102, Boîte Courrier number 16, 9 Quai St Bernard, 75005 Paris, France
3 Récepteurs et Cognition, CNRS URA 2182, Institut Pasteur, 25 Rue du Dr Roux, 75724 Paris Cedex 15, France
4 Department of Cell Physiology and Metabolism, University of Geneva, 1 Rue Michel Servet, 1211 Geneva 4, Switzerland
5 BioCell Interface, Rue de la Serre 91, CP 131, 2301 La Chaux-de-Fonds, Switzerland
6 These authors contributed equally to this study
To whom correspondence should be addressed
Pierre Vincent Tel: +33 1 44 27 25 88; Fax: +33 1 44 27 25 84; E-mail: pierre.vincent@snv.jussieu.fr
Paolo Meda Tel: +41 22 379 52 10; Fax: +41 22 379 52 60; E-mail: paolo.meda@medecine.unige.ch
Received 26 October 2005; Accepted 28 July 2006; Published online 29 September 2006.
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Abstract
Only a few methods permit researchers to study selected regions of the central and peripheral nervous systems with a spatial and time resolution sufficient to image the function of neural structures. Usually, these methods cannot analyse deep-brain regions and a high-resolution method, which could repeatedly probe dynamic processes in any region of the central and peripheral nervous systems, is much needed. Here, we show that fibred fluorescence microscopy—which uses a small-diameter fibre-optic probe to provide real-time images—has the spatial resolution to image various neural structures in the living animal, the consistency needed for a sequential, quantitative evaluation of axonal degeneration/regeneration of a peripheral nerve, and the sensitivity to detect calcium transients on a sub-second timescale. These unique features should prove useful in many physiological studies requiring the in situ functional imaging of tissues in a living animal.
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