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EMBO reports 7, 10, 1040–1045 (2006)
doi:10.1038/sj.embor.7400772
AOP Published online: 28 July 2006
The CRY box: a second APCcdh1-dependent degron in mammalian cdc20
Alexandra Reis1, Mark Levasseur1, Heng-Yu Chang1, David J Elliott2 & Keith T Jones1
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1 Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle-upon-Tyne, Newcastle-upon-Tyne NE2 4HH, UK
2 Institute of Human Genetics, International Centre for Life, University of Newcastle-upon-Tyne, Central Parkway, Newcastle-upon-Tyne NE1 3BZ, UK
To whom correspondence should be addressed
Keith T Jones Tel: +44 191 222 6963; Fax: +44 191 222 7424; E-mail: k.t.jones@newcastle.ac.uk
Received 11 April 2006; Accepted 6 July 2006; Published online 28 July 2006.
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Abstract
Cdc20 and cdh1 are coactivators of the anaphase-promoting complex (APC). APCcdc20 is necessary for the metaphase–anaphase transition and, at the end of mitosis, vertebrate cdc20 itself becomes a target for degradation through KEN-box-dependent APCcdh1 activity. By studying the degradation of fluorescent protein chimaeras in mammalian oocytes and early embryos, we found that cdc20 was degraded through two independent degradation signals (degrons), the KEN box and a newly described CRY box. In both oocytes and G1-stage embryos, the rate of degradation through the CRY box was greater than through the KEN box, although both were mediated by APCcdh1. Thus, mammalian oocytes and embryos have the capacity to recognize two degrons in cdc20.
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