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EMBO reports 6, 11, 1058–1063 (2005)
doi:10.1038/sj.embor.7400529
AOP Published online: 9 September 2005
A pre-anaphase role for a Cks/Suc1 in acentrosomal spindle formation of Drosophila female meiosis
Neil J Pearson, C Fiona Cullen, Nikola S Dzhindzhev & Hiroyuki Ohkura
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Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh EH9 3JR, UK
To whom correspondence should be addressed
Hiroyuki Ohkura Tel: +44 131 650 7094; Fax: +44 131 650 8650; E-mail: h.ohkura@ed.ac.uk
Received 4 April 2005; Accepted 15 August 2005; Published online 9 September 2005.
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Abstract
Conventional centrosomes are absent from a female meiotic spindle in many animals. Instead, chromosomes drive spindle assembly, but the molecular mechanism of this acentrosomal spindle formation is not well understood. We have screened female sterile mutations for defects in acentrosomal spindle formation in Drosophila female meiosis. One of them, remnants (rem), disrupted bipolar spindle morphology and chromosome alignment in non-activated oocytes. We found that rem encodes a conserved subunit of Cdc2 (Cks30A). As Drosophila oocytes arrest in metaphase I, the defect represents a new Cks function before metaphase–anaphase transition. In addition, we found that the essential pole components, Msps and D-TACC, were often mislocalized to the equator, which may explain part of the spindle defect. We showed that the second cks gene cks85A, in contrast, has an important role in mitosis. In conclusion, this study describes a new pre-anaphase role for a Cks in acentrosomal meiotic spindle formation.
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