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EMBO reports 6, 1, 77–82 (2005)
doi:10.1038/sj.embor.7400309
Published online: 10 December 2004
Transcription regulation by the adaptor protein Fe65 and the nucleosome assembly factor SET
Francesca Telese1, Paola Bruni1, Aldo Donizetti1, Davide Gianni1, 2, Chiara D'Ambrosio3, Andrea Scaloni3, Nicola Zambrano1, Michael G Rosenfeld2 & Tommaso Russo1
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1 CEINGE Biotecnologie Avanzate, Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Via Comunale Margherita 482, 80131 Napoli, Italy
2 Howard Hughes Medical Institute, University of California, 9500 Gilman Drive, San Diego, La Jolla, California 92093, USA
3 Proteomics and Mass Spectrometry Laboratory, ISPAAM, CNR, Via Argine 1085, 80147 Naples, Italy
To whom correspondence should be addressed
Tommaso Russo Tel: +39 081 372 2863; Fax: +39 081 372 2808; E-mail: russot@dbbm.unina.it
Received 5 July 2004; Accepted 10 November 2004; Published online 10 December 2004.
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Abstract
Fe65 protein interacts with the cytosolic domain of the amyloid precursor APP. Its possible involvement in gene regulation is suggested by numerous observations, including those demonstrating that it activates transcription. Here, we show that the Fe65 transcription activation domain overlaps with the WW domain of Fe65 and binds to the nucleosome assembly factor SET. This protein is required for the Fe65-mediated transactivation of a reporter gene. Two-step chromatin immunoprecipitation experiments demonstrate that a complex including Fe65/AICD/Tip60 and SET is associated with the KAI1 gene promoter. Suppression of SET levels by RNA interference shows that this protein is required for full levels of basal transcription of the KAI1 gene. These results further support the function of Fe65 and APP in gene regulation and show a new role for the SET factor.
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