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EMBO reports 1, 3, 282–286 (2000)
doi:10.1093/embo-reports/kvd045
Published online: September 2000
Figure 3
The EXT1/EXT2 tumor suppressors: catalytic activities and role in heparan sulfate biosynthesis
Claire Senay, Thomas Lind, Kumi Muguruma, Yuko Tone, Hiroshi Kitagawa, Kazuyuki Sugahara, Kerstin Lidholt, Ulf Lindahl & Marion Kusche-Gullberg
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| Endo H treatment of EXT-proteins expressed in Pichia. Membrane fractions from yeast transformed separately with myc-his tagged EXT1 or EXT2 or cotransformed with EXT1 and EXT2 (EXT1/2) were incubated in the absence or presence of Endo H and analyzed by 7.5% SDS–PAGE followed by western blotting with an antibody to the polyhistidine-tag. Transformed clones are identified at the top. The kilodalton values of protein molecular mass markers are indicated. Mock, transformed with vector alone.
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