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EMBO reports 1, 3, 282–286 (2000)
doi:10.1093/embo-reports/kvd045
Published online: September 2000
Figure 1
The EXT1/EXT2 tumor suppressors: catalytic activities and role in heparan sulfate biosynthesis
Claire Senay, Thomas Lind, Kumi Muguruma, Yuko Tone, Hiroshi Kitagawa, Kazuyuki Sugahara, Kerstin Lidholt, Ulf Lindahl & Marion Kusche-Gullberg
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Scheme of EXT constructs. (A) With transmembrane domain (TM, black box). For expression in yeast, EXT1 and EXT2 cDNA constructs were inserted into the pPICZB vector (Invitrogen). For expression in COS-1 cells, EXT1 and EXT2 were ligated into the pcDNA3.1 expression vector (Invitrogen). (B) Without transmembrane domain (SP, signal peptide, open box). EXT constructs (sEXT) lacking the first N-terminal 32 (sEXT1-GFP) or 48 (sEXT2myc-his) amino acid residues, respectively, were ligated into the pPICZ C vector (Invitrogen). sEXT1protA and sEXT2protA constructs lacking the first N-terminal 43 or 55 amino acid residues, respectively, were subcloned into pGIR201protA (Kitagawa and Paulson, 1994) and subsequently inserted into the expression vector pEF-BOS. N and C, N- and C-terminus, respectively.
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