Article
- The EMBO Journal (2009) 28, 810 - 820
- doi:10.1038/emboj.2009.31
Published online: 12 February 2009
There is a Have you seen ...? (April 2009) associated with this Article.
Subject Categories:
Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres
Bettina A Moser1, Lakxmi Subramanian1, Ya-Ting Chang1, Chiaki Noguchi2, Eishi Noguchi2 and Toru M Nakamura1
- Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, Chicago, IL, USA
- Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA, USA
Correspondence to:
Toru M Nakamura, Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, 900 S. Ashland Ave. MC669, Chicago, IL 60607, USA. Tel.: +1 312 996 1988; Fax: +1 312 996 1988; E-mail: nakamut@uic.edu
Received 24 October 2008; Accepted 20 January 2009
Abstract
To maintain genomic integrity, telomeres must undergo switches from a protected state to an accessible state that allows telomerase recruitment. To better understand how telomere accessibility is regulated in fission yeast, we analysed cell cycle-dependent recruitment of telomere-specific proteins (telomerase Trt1, Taz1, Rap1, Pot1 and Stn1), DNA replication proteins (DNA polymerases, MCM, RPA), checkpoint protein Rad26 and DNA repair protein Nbs1 to telomeres. Quantitative chromatin immunoprecipitation studies revealed that MCM, Nbs1 and Stn1 could be recruited to telomeres in the absence of telomere replication in S-phase. In contrast, Trt1, Pot1, RPA and Rad26 failed to efficiently associate with telomeres unless telomeres are actively replicated. Unexpectedly, the leading strand DNA polymerase 
(Pol) arrived at telomeres earlier than the lagging strand DNA polymerases 
(Pol) and 
(Pol). Recruitment of RPA and Rad26 to telomeres matched arrival of DNA Pol, whereas S-phase specific recruitment of Trt1, Pot1 and Stn1 matched arrival of DNA Pol. Thus, the conversion of telomere states involves an unanticipated intermediate step where lagging strand synthesis is delayed until telomerase is recruited.
Keywords:
- cell cycle,
- DNA polymerase,
- DNA replication,
- pot1,
- telomerase
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated
REVIEWS
Nature Reviews Molecular Cell Biology Review (01 Oct 2007)
NEWS AND VIEWS
Nature News and Views (13 Jan 2000)
Nature Structural & Molecular Biology News and Views (01 Mar 2007)
RESEARCH
Met-driven invasive growth involves transcriptional regulation of Arhgap12
Oncogene Original Article
RPA-like proteins mediate yeast telomere function
Nature Structural & Molecular Biology Article (01 Mar 2007)
