Article
- The EMBO Journal (2009) 28, 1562 - 1575
- doi:10.1038/emboj.2009.111
Published online: 23 April 2009
Subject Category:
Lte1, Cdc14 and MEN-controlled Cdk inactivation in yeast coordinate rDNA decompaction with late telophase progression
Elisa Varela1,a, Kenji Shimada1, Thierry Laroche2, Didier Leroy3 and Susan M Gasser1
- Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
- EPFL, BioImaging and Optics Platform, Lausanne, Switzerland
- Medicines for Malaria Venture, International Center Cointrin, Geneva, Switzerland
Correspondence to:
Susan M Gasser, Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, Basel 4058, Switzerland. Tel.: +41 61 697 7255; Fax: +41 61 697 3976; E-mail: susan.gasser@fmi.ch
aPresent address: Spanish National Cancer Research Centre (CNIO), Melchor Fernández Almagro 3, E-28029 Madrid
Received 3 October 2008; Accepted 26 March 2009
Abstract
The mechanism of chromatin compaction in mitosis has been well studied, while little is known about what controls chromatin decompaction in early G1 phase. We have localized the Condensin subunit Brn1 to a compact spiral of rDNA in mitotic budding yeast cells. Brn1 release and the resulting rDNA decompaction in late telophase coincided with mitotic spindle dissociation, and occurred asymmetrically (daughter cells first). We immunoprecipitated the GTP-exchange factor Lte1, which helps activate the mitotic exit network (MEN) in anaphase, with mitotic Brn1. In lte
cells Brn1 release was delayed, even at temperatures that do not impair mitotic exit. Mutations in MEN pathway components that act downstream of Lte1 similarly delayed rDNA decompaction. We found that Brn1 release in wild-type cells coincided with the release of Cdc14 phosphatase from the nucleolus and with mitotic CDK inactivation, yet it could be selectively delayed by perturbation of the MEN pathway. This may argue that different levels of Cdk inactivation control spindle disassembly and chromatin decompaction. Mutation of lte1 also impaired rotation of the nucleus in early G1.
Keywords:
- Brn1,
- Cdc14,
- chromatin decondensation,
- Condensin,
- mitotic exit network (MEN)
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