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  • The EMBO Journal (2009) 28, 1466 - 1478
  • doi:10.1038/emboj.2009.92

Published online: 16 April 2009

Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replication

Fei-Long Meng1, Yan Hu1, Ning Shen1, Xia-Jing Tong1, Jianyong Wang1, Jianping Ding1 and Jin-Qiu Zhou1

  1. The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China

Correspondence to:

Jin-Qiu Zhou, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China. Tel.: +86 21 5492 1076; Fax: +86 21 5492 1075; E-mail: jqzhou@sibs.ac.cn

Received 16 October 2008; Accepted 17 March 2009

In budding yeast Saccharomyces cerevisiae, telomere length maintenance involves a complicated network as more than 280 telomere maintenance genes have been identified in the nonessential gene deletion mutant set. As a supplement, we identified additional 29 telomere maintenance genes, which were previously taken as essential genes. In this study, we report a novel function of Sua5p in telomere replication. Epistasis analysis and telomere sequencing show that sua5Delta cells display progressively shortened telomeres at early passages, and Sua5 functions downstream telomerase recruitment. Further, biochemical, structural and genetic studies show that Sua5p specifically binds single-stranded telomeric (ssTG) DNA in vitro through a distinct DNA-binding region on its surface, and the DNA-binding ability is essential for its telomere function. Thus, Sua5p represents a novel ssTG DNA-binding protein and positively regulates the telomere length in vivo.

  • Keywords:

    • DNA binding,
    • OB-fold,
    • Sua5,
    • telomerase,
    • telomere